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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CYTIP Antibody (aa2‑14) IHC‑plus™ LS‑B7390 Best Seller
Note: This antibody replaces LS-C54689
CYTIP antibody LS-B7390 is an unconjugated goat polyclonal antibody to CYTIP from human and monkey. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B7390-50
50 µg (0.5 mg/ml)
$425

Popular CYTIP Products

Anti-Cybr Antibody - Western Blot. Western blot of anti-Cybr antibody shows detection of recombinant Cybr using anti-HA tag antibody after immunoprecipitation using anti-Cybr. Lane 5 contains lysate from control HEK293 cells. Lane 6 shows IP/WB of HEK293 cells expressing Cybr-HA. Lane 7 and 8 are loaded similarly to lanes 5 and 6 but were immunoprecipitated with anti-HA. Lane 9 contains marker. Lane 10 contains purified CybrHA. Personal Communication, V. Coppola, NCI, Bethesda, MD.
Species: Mouse, Human, Rat
Applications: Western blot, Immunoprecipitation, ELISA
Anti-Cybr Antibody - Western Blot. Western blot of affinity purified anti-Cybr antibody shows detection of endogenous Cybr from mouse splenocytes using anti-Cybr antibody to immunoprecipitate and western blot (lanes 1-3). Lane 1 shows reactivity of pre-immune sera. Lane 2 shows endogenous Cybr detected with antibody. Lane 3 shows no band detected in lysates prepared from splenocytes of Cybr knock-out mouse. Lane 5 shows direct Western blot of wt splenocytes. Lane 6 shows direct Western blot of knock out mouse. Personal Communication, V. Coppola, NCI, Bethesda, MD.
Species: Mouse, Rat
Applications: Western blot, ELISA
Western blot using the affinity purified anti-Cybr antibody shows detection of endogenous Cybr from mouse splenocytes using anti-Cybr antibody to immunoprecipitate and western blot (lanes 1-3). Lane 1 shows reactivity of pre-immune sera. Lane 2 shows endogenous Cybr detected with antibody. Lane 3 shows no band detected in lysates prepared from splenocytes of Cybr knock-out mouse. Lane 5 shows direct western blot of wt splenocytes. Lane 6 shows direct western blot of knock out mouse.
Species: Mouse, Rat
Applications: Western blot, ELISA
 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human
Applications: Western blot

Product Description

CYTIP antibody LS-B7390 is an unconjugated goat polyclonal antibody to CYTIP from human and monkey. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About CYTIP
CYTIP contains 2 leucine zipper domains and a putative C-terminal nuclear targeting signal, but does not have any hydrophobic regions. This protein is expressed weakly in resting NK and T cells. The encoded protein modulates the activation of ARF genes by CYTH1. This protein interacts with CYTH1 and SNX27 proteins and may act to sequester CYTH1 protein in the cytoplasm. O60759 NM_004288 NP_004279.3

CYTIP Antibody, B3-1 Antibody, CASP Antibody, Cbp HE Antibody, CYTHIP Antibody, Cytohesin binder and regulator Antibody, Cytohesin binding protein HE Antibody, Cytohesin-binding protein HE Antibody, CYBR Antibody, Cytohesin-interacting protein Antibody, HE Antibody, PSCDBP Antibody


Specifications

Target
Human CYTIP
Host
Goat
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Predicted
Dog, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CYTIP antibody was raised against synthetic peptide SLQRLLQHSSNGN-C from the N-terminus of human CYTIP (NP_004279.3). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Dog, Horse (92%).
Blocking Peptide
LS-E25321 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B7390. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa2-14
Specificity
Human CYTIP.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry

Anti-CYTIP antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7390 concentration 5 ug/ml.
Anti-CYTIP antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7390 concentration 5 ug/ml.

Western blot

Antibody staining (1 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (1 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Immunohistochemistry

Anti-CYTIP antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7390 concentration 5 ug/ml.
Anti-CYTIP antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7390 concentration 5 ug/ml.

Western blot

Antibody staining (1 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (1 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Immunohistochemistry

Anti-CYTIP antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7390 concentration 5 ug/ml.
Anti-CYTIP antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7390 concentration 5 ug/ml.

Western blot

Antibody staining (1 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (1 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Immunohistochemistry

Anti-CYTIP antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7390 concentration 5 ug/ml.
Anti-CYTIP antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7390 concentration 5 ug/ml.

Western blot

Antibody staining (1 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (1 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Requested From: United States
Date Requested: 9/23/2018

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