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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CYSLT2 / CYSLTR2 Antibody (Extracellular Domain, APC) IHC‑plus™ LS‑A102654
CYSLTR2 antibody LS-A102654 is an APC-conjugated rabbit polyclonal antibody to CYSLTR2 (CYSLT2) from human and horse. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A102654-100
100 µl
$650
Description
CYSLTR2 antibody LS-A102654 is an APC-conjugated rabbit polyclonal antibody to CYSLTR2 (CYSLT2) from human and horse. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human CYSLT2 / CYSLTR2
Host
Rabbit
Reactivity
Human, Horse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Allophycocyanin. Also available Unconjugated or conjugated with AP, Biotin, FITC, HRP, PE.  (This antibody is custom conjugated upon order. Following conjugation, the final antibody concentration will be determined and is typically > 0.5 mg/ml.)
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 17 amino acid peptide from 1st extracellular domain of human CYSLTR2 / CYSLT2. Percent identity with other species by BLAST analysis: Human, Gibbon, Marmoset, Horse (100%); Gorilla, Monkey, Mouse, Panda (94%); Dog, Bovine, Hamster, Elephant, Rabbit, Opossum (88%); Pig (82%).
Epitope
Extracellular Domain
Specificity
Human CYSLTR2 / CYSLT2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GPR109B (59%), OXGR1 (59%), GPR81 (59%).
Usage
Immunohistochemistry: LS-A2255 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A2255 was determined to be 10 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About CYSLT2 / CYSLTR2
Q9NS75 NM_020377 NP_065110.1

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Immunohistochemistry

Anti-CYSLTR2 / CYSLT2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CYSLTR2 / CYSLT2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CYSLTR2 / CYSLT2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CYSLTR2 / CYSLT2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CYSLTR2 / CYSLT2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CYSLTR2 / CYSLT2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-CYSLTR2 / CYSLT2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CYSLTR2 / CYSLT2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: United States
Date Requested: 12/12/2018
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