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Antibodies > CYP8B1 > LS-C675073
 
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Catalog Number Size Price
LS-C675073-50 50 µg $294 
LS-C675073-100 100 µg $360 
CYP8B1 Antibody - Immunohistochemistry image at a dilution of 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CYP8B1 Antibody - Immunohistochemistry image at a dilution of 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CYP8B1 Antibody - Immunofluorescence staining of HepG2 cells at a dilution of 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
CYP8B1 Antibody - Positive Western Blot detected in Rat kidney tissue. All lanes: CYP8B1 antibody at 6.4 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 59 KDa. Observed band size: 59 KDa
CYP8B1 Antibody - Immunohistochemistry image at a dilution of 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CYP8B1 Antibody - Immunohistochemistry image at a dilution of 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CYP8B1 Antibody - Immunofluorescence staining of HepG2 cells at a dilution of 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
CYP8B1 Antibody - Positive Western Blot detected in Rat kidney tissue. All lanes: CYP8B1 antibody at 6.4 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 59 KDa. Observed band size: 59 KDa
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Polyclonal Rabbit anti‑Human CYP8B1 Antibody (IHC, IF, WB) LS‑C675073

Polyclonal Rabbit anti‑Human CYP8B1 Antibody (IHC, IF, WB) LS‑C675073

Submit Review LSBio Guarantee
Antibody:
CYP8B1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
HRP FITC Biotin
Price
Catalog Number
$294
LS-C675073-50
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CYP8B1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
HRP FITC Biotin

Specifications

Description
CYP8B1 antibody LS-C675073 is an unconjugated rabbit polyclonal antibody to human CYP8B1. Validated for IF, IHC and WB.
Target
Human CYP8B1
Synonyms
CYP8B1 | CYP12 | Cytochrome P450 8B1 | CP8B | Sterol 12-alpha-hydroxylase | Sterol 12alpha-hydroxylase | CYPVIIIB1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with HRP, FITC, Biotin.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant Human 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase protein (303-440AA)
Applications
  • IHC - Paraffin
  • Immunofluorescence
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CYP8B1
Q9UNU6 NM_004391 NP_004382.2

Publications (0)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/17/2024
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