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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CYP7A1 Antibody (aa27-58)

CYP7A1 antibody LS-C392646 is an unconjugated rabbit polyclonal antibody to human CYP7A1. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C392646-80
80 µl
$205
Description
CYP7A1 antibody LS-C392646 is an unconjugated rabbit polyclonal antibody to human CYP7A1. Validated for Peptide-ELISA and WB.
Target
Human CYP7A1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay
Epitope
aa27-58
Specificity
This CYP7A1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 27-58 amino acids from the N-terminal region of human CYP7A1.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
About CYP7A1
P22680 NM_000780 NP_000771.2

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Images

Western blot

All lanes: Anti-CYP7A1 Antibody (N-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysates. Lane 2: HeLa whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-CYP7A1 Antibody (N-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysates. Lane 2: HeLa whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot

All lanes: Anti-CYP7A1 Antibody (N-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysates. Lane 2: HeLa whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-CYP7A1 Antibody (N-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysates. Lane 2: HeLa whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot

All lanes: Anti-CYP7A1 Antibody (N-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysates. Lane 2: HeLa whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-CYP7A1 Antibody (N-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysates. Lane 2: HeLa whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot

All lanes: Anti-CYP7A1 Antibody (N-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysates. Lane 2: HeLa whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-CYP7A1 Antibody (N-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysates. Lane 2: HeLa whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Requested From: United States
Date Requested: 1/22/2019
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