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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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CYP7A1 Antibody (aa27‑58) LS‑C392646
CYP7A1 antibody LS-C392646 is an unconjugated rabbit polyclonal antibody to human CYP7A1. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C392646-80
80 µl
$205

Popular CYP7A1 Products

Western blot of CYP7A1 Antibody in K562(lane 1), HepG2(lane 2) cell line lysates (35 ug/lane). CYP7A1 (arrow) was detected using the purified antibody.
Species: Human
Applications: Western blot
Western blot of recombinant CYP7A1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: IHC, Western blot
Antibody
Species: Human
Applications: Western blot
CYP7A1 antibody. IHC(P): Human Liver Cancer Tissue.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
CYP7A1 antibody Western blot. All lanes: Anti CYP7A1 at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: Mouse Liver Tissue Lysate at 50 ug. Predicted band size: 58 kD. Observed band size: 58 kD.
Species: Human, Mouse, Rat
Applications: Western blot

Product Description

CYP7A1 antibody LS-C392646 is an unconjugated rabbit polyclonal antibody to human CYP7A1. Validated for Peptide-ELISA and WB.
About CYP7A1
CYP7A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. P22680 NM_000780 NP_000771.2

CYP7A1 Antibody, Cytochrome P450 7A1 Antibody, Cyp7a Antibody, CYPVII Antibody, CYP7 Antibody, CP7A Antibody


Specifications

Target
Human CYP7A1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay
Epitope
aa27-58
Specificity
This CYP7A1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 27-58 amino acids from the N-terminal region of human CYP7A1.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Western blot

All lanes: Anti-CYP7A1 Antibody (N-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysates. Lane 2: HeLa whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-CYP7A1 Antibody (N-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysates. Lane 2: HeLa whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot

All lanes: Anti-CYP7A1 Antibody (N-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysates. Lane 2: HeLa whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-CYP7A1 Antibody (N-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysates. Lane 2: HeLa whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot

All lanes: Anti-CYP7A1 Antibody (N-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysates. Lane 2: HeLa whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-CYP7A1 Antibody (N-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysates. Lane 2: HeLa whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot

All lanes: Anti-CYP7A1 Antibody (N-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysates. Lane 2: HeLa whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-CYP7A1 Antibody (N-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysates. Lane 2: HeLa whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Requested From: United States
Date Requested: 8/14/2018

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