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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CYP7A1 Antibody (aa109‑383, APC, Cy7) LS‑C731986
CYP7A1 antibody LS-C731986 is an APC, Cy7-conjugated rabbit polyclonal antibody to rat CYP7A1. Validated for IHC and WB.
Catalog
Size
Price
LS-C731986-20
20 µl
$285
LS-C731986-100
100 µl
$465
LS-C731986-200
200 µl
$650
LS-C731986-1
1 ml
$1,620
LS-C731986-10
10 ml
$6,480

Popular CYP7A1 Products

CYP7A1 antibody. IHC(P): Human Liver Cancer Tissue.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: Western blot
Western blot of recombinant CYP7A1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: IHC, Western blot
CYP7A1 antibody Western blot. All lanes: Anti CYP7A1 at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: Mouse Liver Tissue Lysate at 50 ug. Predicted band size: 58 kD. Observed band size: 58 kD.
Species: Human, Mouse, Rat
Applications: Western blot
Western blot of CYP7A1 Antibody in K562(lane 1), HepG2(lane 2) cell line lysates (35 ug/lane). CYP7A1 (arrow) was detected using the purified antibody.
Species: Human
Applications: Western blot

Product Description

LifeSpan Antibody

Specifications

Target
Rat CYP7A1
Synonyms
CYP7A1, Cytochrome P450 7A1, Cyp7a, CYPVII, CYP7, CP7A
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Allophycocyanin, Cy7. Also available Unconjugated or conjugated with Biotin, FITC, Biotin, FITC, Cy3, HRP, APC, PE.
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Modifications
Unmodified
Applications
  • IHC
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CYP7A1 antibody was raised against recombinant CYP7A1 (Phe109-Lys383) expressed in E. coli.
Epitope
aa109-383
Specificity
The antibody is a rabbit polyclonal antibody raised against CYP7A1. It has been selected for its ability to recognize CYP7A1 in immunohistochemical staining and western blotting.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months.
Restrictions
For research use only.
About CYP7A1
CYP7A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. P22680 NM_000780 NP_000771.2


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Images

Western blot

Western blot of recombinant CYP7A1.
Western blot of recombinant CYP7A1.

Western blot

Western blot of recombinant CYP7A1.
Western blot of recombinant CYP7A1.

Western blot

Western blot of recombinant CYP7A1.
Western blot of recombinant CYP7A1.

Western blot

Western blot of recombinant CYP7A1.
Western blot of recombinant CYP7A1.

Requested From: United States
Date Requested: 10/15/2018

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