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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CYP51A1 / CYP51 Antibody (aa474‑503) LS‑C167242
CYP51 antibody LS-C167242 is an unconjugated rabbit polyclonal antibody to human CYP51 (CYP51A1). Validated for IHC and WB.
Catalog
Size
Price
LS-C167242-400
400 µl
$305

Popular CYP51A1 / CYP51 Products

Antibody
Species: Human, Rat
Applications: IHC, Western blot
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with CYP51A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Immunohistochemistry of paraffin-embedded Mouse kidney tissue.
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
Immunohistochemistry of paraffin-embedded human testis tissue at dilution of 1:100
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

CYP51 antibody LS-C167242 is an unconjugated rabbit polyclonal antibody to human CYP51 (CYP51A1). Validated for IHC and WB.
About CYP51A1 / CYP51
CYP51A1 / CYP51 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Q16850 NM_000786 NP_000777.1

CYP51A1 Antibody, Cytochrome P450-14DM Antibody, CYPLI Antibody, Cytochrome P450LI Antibody, Cytochrome P450 51A1 Antibody, Cytochrome P45014DM Antibody, p450L1 Antibody, LDM Antibody, Sterol 14-alpha demethylase Antibody, CP51 Antibody, CYP51 Antibody, CYPL1 Antibody, p450-14DM Antibody


Specifications

Target
Human CYP51A1 / CYP51
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Pig (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E9826 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C167242. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa474-503
Specificity
This CYP51A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 474-503 amino acids from the C-terminal region of human CYP51A1.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.

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Images

Immunohistochemistry

Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with CYP51A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with CYP51A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of CYP51A1 Antibody in HL-60 cell line lysates (35 ug/lane). CYP51A1 (arrow) was detected using the purified antibody.
Western blot of CYP51A1 Antibody in HL-60 cell line lysates (35 ug/lane). CYP51A1 (arrow) was detected using the purified antibody.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with CYP51A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with CYP51A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of CYP51A1 Antibody in HL-60 cell line lysates (35 ug/lane). CYP51A1 (arrow) was detected using the purified antibody.
Western blot of CYP51A1 Antibody in HL-60 cell line lysates (35 ug/lane). CYP51A1 (arrow) was detected using the purified antibody.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with CYP51A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with CYP51A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of CYP51A1 Antibody in HL-60 cell line lysates (35 ug/lane). CYP51A1 (arrow) was detected using the purified antibody.
Western blot of CYP51A1 Antibody in HL-60 cell line lysates (35 ug/lane). CYP51A1 (arrow) was detected using the purified antibody.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with CYP51A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with CYP51A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of CYP51A1 Antibody in HL-60 cell line lysates (35 ug/lane). CYP51A1 (arrow) was detected using the purified antibody.
Western blot of CYP51A1 Antibody in HL-60 cell line lysates (35 ug/lane). CYP51A1 (arrow) was detected using the purified antibody.

Requested From: United States
Date Requested: 9/22/2018

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