CYP51 antibody LS-C135728 is an unconjugated rabbit polyclonal antibody to CYP51 (CYP51A1) from human, bat, chicken and other species. Validated for WB.
Human CYP51A1 / CYP51
Human, Monkey, Bat, Dog, Guinea pig, Horse, Pig, Chicken, Xenopus
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
- Western blot (0.2 - 1 µg/ml)
- (applications tested for the base form of this product only)
CYP51A1 / CYP51 antibody was raised against synthetic peptide located between aa112-161 of human CYP51A1 (Q16850, NP_000777). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Elephant, Dog, Bat, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus, Lizard, Xenopus (100%); Mouse, Rat, Bovine, Rabbit, Stickleback, Zebrafish (92%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C135728. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Western Blot: Suggested dilution at 0.25 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.