CYP51 antibody LS-C135728 is an unconjugated rabbit polyclonal antibody to CYP51 (CYP51A1) from human, bat, chicken and other species. Validated for WB.
Human, Monkey, Bat, Dog, Guinea pig, Horse, Pig, Chicken, Xenopus
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
CYP51A1 / CYP51 antibody was raised against synthetic peptide located between aa112-161 of human CYP51A1 (Q16850, NP_000777). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Elephant, Dog, Bat, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus, Lizard, Xenopus (100%); Mouse, Rat, Bovine, Rabbit, Stickleback, Zebrafish (92%).
Western Blot: Suggested dilution at 0.25 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
PBS, 0.09% sodium azide, 2% sucrose
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.