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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CYP2J2 Antibody (aa156‑183) LS‑C100653
CYP2J2 antibody LS-C100653 is an unconjugated rabbit polyclonal antibody to human CYP2J2. Validated for IHC and WB.
Catalog
Size
Price
LS-C100653-400
400 µl
$305

Popular CYP2J2 Products

Immunohistochemical analysis of Cytochrome P450 2J2 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
Western blot of HT29 cell lysate using Cytochrome P450 2J2 Antibody
Species: Human
Applications: IHC, ICC, Immunofluorescence, Western blot
Immunohistochemistry analysis of paraffin-embedded human heart tissue, using Cytochrome P450 2J2 Antibody. The picture on the right is blocked with the synthesized peptide.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Western blot of CYP2J2 antibody
Species: Human, Monkey
Applications: IHC, Immunofluorescence, Western blot, ELISA
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CYP2J2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry

Product Description

CYP2J2 antibody LS-C100653 is an unconjugated rabbit polyclonal antibody to human CYP2J2. Validated for IHC and WB.
About CYP2J2
This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools. P51589 NM_000775 NP_000766.2

CYP2J2 Antibody, Arachidonic acid epoxygenase Antibody, CYPIIJ2 Antibody, Cytochrome p450 IIj2 Antibody, CPJ2 Antibody, Cytochrome P450 2J2 Antibody


Specifications

Target
Human CYP2J2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E8966 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C100653. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa156-183
Specificity
This CYP2J2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-183 amino acids from the Central region of human CYP2J2.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.

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Images

Immunohistochemistry

CYP2J2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.
CYP2J2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of anti-CYP2J2 Antibody in CEM cell line lysates (35 ug/lane). CYP2J2 (arrow) was detected using the purified antibody.
Western blot of anti-CYP2J2 Antibody in CEM cell line lysates (35 ug/lane). CYP2J2 (arrow) was detected using the purified antibody.

Immunohistochemistry

CYP2J2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.
CYP2J2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of anti-CYP2J2 Antibody in CEM cell line lysates (35 ug/lane). CYP2J2 (arrow) was detected using the purified antibody.
Western blot of anti-CYP2J2 Antibody in CEM cell line lysates (35 ug/lane). CYP2J2 (arrow) was detected using the purified antibody.

Immunohistochemistry

CYP2J2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.
CYP2J2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of anti-CYP2J2 Antibody in CEM cell line lysates (35 ug/lane). CYP2J2 (arrow) was detected using the purified antibody.
Western blot of anti-CYP2J2 Antibody in CEM cell line lysates (35 ug/lane). CYP2J2 (arrow) was detected using the purified antibody.

Immunohistochemistry

CYP2J2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.
CYP2J2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of anti-CYP2J2 Antibody in CEM cell line lysates (35 ug/lane). CYP2J2 (arrow) was detected using the purified antibody.
Western blot of anti-CYP2J2 Antibody in CEM cell line lysates (35 ug/lane). CYP2J2 (arrow) was detected using the purified antibody.

Requested From: United States
Date Requested: 9/22/2018

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