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Antibodies > CYP21A2 > LS-C355985
 
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Catalog Number Size Price
LS-C355985-100 100 µl (1 mg/ml) $299 
CYP21A2 Antibody - Immunohistochemical analysis of Cytochrome P450 21A2 staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
CYP21A2 Antibody - Immunofluorescent analysis of Cytochrome P450 21A2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CYP21A2 Antibody - Western blot analysis of Cytochrome P450 21A2 expression in HeLa (A) whole cell lysates.
CYP21A2 Antibody - Immunohistochemical analysis of Cytochrome P450 21A2 staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
CYP21A2 Antibody - Immunofluorescent analysis of Cytochrome P450 21A2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CYP21A2 Antibody - Western blot analysis of Cytochrome P450 21A2 expression in HeLa (A) whole cell lysates.
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Polyclonal Rabbit anti‑Human CYP21A2 Antibody (Internal, IHC, IF, WB) LS‑C355985

Polyclonal Rabbit anti‑Human CYP21A2 Antibody (Internal, IHC, IF, WB) LS‑C355985

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Antibody:
CYP21A2 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C355985-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CYP21A2 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CYP21A2 antibody LS-C355985 is an unconjugated rabbit polyclonal antibody to human CYP21A2 (Internal). Validated for ICC, IF, IHC and WB.
Target
Human CYP21A2
Synonyms
CYP21A2 | 21-OHase | CAH1 | CYP21B | Cytochrome P450-C21 | Cytochrome P450 21 | Cytochrome P450 XXI | p450c21B | CA21H | CYP21 | Cytochrome P-450c21 | Cytochrome P450-C21B | Steroid 21-hydroxylase | Steroid 21-monooxygenase
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human Cytochrome P450 21A2.
Epitope
Internal
Specificity
Recognizes endogenous levels of Cytochrome P450 21A2 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CYP21A2
P08686 NM_000500 NP_000491.4

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CYP21A2 Antibody (aa314‑363) LS‑B9758
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CYP21A2 Antibody - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ CYP21A2 Antibody (aa216‑246) LS‑B12176
Species: Human
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CYP21A2 Antibody - Anti-CYP21A2 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC‑plus™ CYP21A2 Antibody (aa151‑200) LS‑B9248
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Product
CYP21A2 Antibody (aa160‑188, Biotin) LS‑C221964
Species: Human
Applications: Western blot, ELISA
CYP21A2 Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
CYP21A2 Antibody LS‑C805637
Species: Human
Applications: Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
CYP21A2 ELISA Kit
Human CYP21A2 (Sandwich ELISA) ELISA Kit - LS-F11190
Reactivity: Human
Range: 0.312-20 ng/ml
See all CYP21A2 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
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