Target
Human CYP1A1
Synonyms
CYP1A1 | AHH | Aryl hydrocarbon hydroxylase | Cytochrome P450-C | CP11 | CYPIA1 | Cytochrome P450 form 6 | Cytochrome p450 ia1 | Cytochrome P450 1A1 | Cytochrome P450-P1 | p1-450 | p450-C | p450DX | CYP1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence CQEKQLDENANVQ, from the internal region of the protein sequence according to NP_000490.1.
Specificity
This antibody is not expected to cross-react with the similar P450 1A2
Applications
- Western blot (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 85-90kDa band observed in lysates of cell lines A549 and MCF7 (calculated MW of 58.2kDa according to NP_000490.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee