Aromatase antibody LS-B521 is an unconjugated rabbit polyclonal antibody to human Aromatase (CYP19). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Human CYP19 / Aromatase
(tested or 100% immunogen sequence identity)
Bovine, Horse, Rabbit (at least 90% immunogen sequence identity)
Also available conjugated with Biotin
- IHC - Paraffin (10 µg/ml)
- Western blot (1 µg/ml)
- (applications tested for the base form of this product only)
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A synthetic peptide made to a C-terminal portion of the human aromatase protein sequence (between residues 400-502). Percent identity by BLAST analysis: Horse (91%); Bovine, Rabbit (90%); Porcine (83%); Sheep (81%).
Specific for aromatase.
Immunohistochemistry: LS-B521 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B521 was determined to be 10 ug/ml.
Tris-citrate/phosphate buffer, pH 7-8, 0.1% sodium azide.
Store at 4°C. Do not freeze.
For research use only.