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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CYP19 / Aromatase Antibody (aa400‑502) IHC‑plus™ LS‑B521 Best Seller
Note: This antibody replaces LS-C2605
Aromatase antibody LS-B521 is an unconjugated rabbit polyclonal antibody to human Aromatase (CYP19). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Catalog
Size
Price
LS-B521-100
100 µl (1.2 mg/ml)
Unavailable

Popular CYP19 / Aromatase Products

Anti-Aromatase antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2816 concentration 10 ug/ml.
Species: Human, Mouse, Rat, Bovine, Horse, Pig, Rabbit, Sheep, Chicken
Applications: IHC, IHC - Paraffin, Western blot
Anti-Aromatase antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3021 concentration 4 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
IHC of paraffin-embedded human brain tissue, using Cytochrome P450 19A1 Antibody. The picture on the right is treated with the synthesized peptide.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-Aromatase antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7706 dilution 1:100.
Species: Human, Rat, Chicken
Applications: IHC, IHC - Paraffin, Western blot

Product Description

Aromatase antibody LS-B521 is an unconjugated rabbit polyclonal antibody to human Aromatase (CYP19). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
About CYP19 / Aromatase
CYP19 / Aromatase is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. P11511 NM_031226 NP_000094.2

CYP19A1 Antibody, ARO1 Antibody, CYAR Antibody, CPV1 Antibody, CYPXIX Antibody, Cytochrome P-450AROM Antibody, Cytochrome P450 19A1 Antibody, CYP19 Antibody, Estrogen synthase Antibody, Estrogen synthetase Antibody, P-450AROM Antibody, ARO Antibody, Aromatase Antibody


Specifications

Target
Human CYP19 / Aromatase
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Bovine, Horse, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A synthetic peptide made to a C-terminal portion of the human aromatase protein sequence (between residues 400-502). Percent identity by BLAST analysis: Horse (91%); Bovine, Rabbit (90%); Porcine (83%); Sheep (81%).
Epitope
aa400-502
Specificity
Specific for aromatase.
Usage
Immunohistochemistry: LS-B521 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B521 was determined to be 10 ug/ml.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.1% sodium azide.
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (1)

1
Androgen metabolites impact CSF amines and axonal serotonin via MAO-A and -B in male macaques. Bethea CL, Phu K, Kim A, Reddy AP. Neuroscience. 2015 301:576-89. (IHC; Macaque) [Full Text Article] [PubMed:26086546] [PMC:PMC4520442]

Customer Reviews (0)


Images


Immunohistochemistry

Anti-Aromatase antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B521 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Aromatase antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B521 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-Aromatase antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B521 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Aromatase antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B521 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-Aromatase antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B521 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Aromatase antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B521 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-Aromatase antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B521 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Aromatase antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B521 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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