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Antibodies > CYP17 / CYP17A1 > LS-C352100
 
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Catalog Number Size Price
LS-C352100-100 100 µl (1 mg/ml) $299 
CYP17 / CYP17A1 Antibody - Immunohistochemical analysis of Cytochrome P450 17A1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
CYP17 / CYP17A1 Antibody - Immunofluorescent analysis of Cytochrome P450 17A1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CYP17 / CYP17A1 Antibody - Western blot analysis of Cytochrome P450 17A1 expression in HeLa (A); NIH3T3 (B); H9C2 (C); SW13 (D); ES2 (E) whole cell lysates.
CYP17 / CYP17A1 Antibody - Immunohistochemical analysis of Cytochrome P450 17A1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
CYP17 / CYP17A1 Antibody - Immunofluorescent analysis of Cytochrome P450 17A1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CYP17 / CYP17A1 Antibody - Western blot analysis of Cytochrome P450 17A1 expression in HeLa (A); NIH3T3 (B); H9C2 (C); SW13 (D); ES2 (E) whole cell lysates.
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Polyclonal Rabbit anti‑Human CYP17 / CYP17A1 Antibody (Internal, IHC, IF, WB) LS‑C352100

Polyclonal Rabbit anti‑Human CYP17 / CYP17A1 Antibody (Internal, IHC, IF, WB) LS‑C352100

(4.0) Rating
Submit Review (1) Publication(s) LSBio Guarantee
Antibody:
CYP17 / CYP17A1 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C352100-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CYP17 / CYP17A1 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CYP17A1 antibody LS-C352100 is an unconjugated rabbit polyclonal antibody to CYP17A1 (CYP17) (Internal) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC, IP and WB. Cited in 1 publication.
(1) Publication(s)
Target
Human CYP17 / CYP17A1
Synonyms
CYP17A1 | CYP17 | CYPXVII | CPT7 | Cytochrome P450-C17 | Cytochrome P450c17 | p450C17 | Steroid 17-alpha-monooxygenase | Cytochrome P450 17A1 | Cytochrome p450 XVIIA1 | S17AH
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human Cytochrome P450 17A1.
Epitope
Internal
Specificity
Recognizes endogenous levels of Cytochrome P450 17A1 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CYP17 / CYP17A1
P05093 NM_000102 NP_000093.1

LSBio Ratings

CYP17 / CYP17A1 Antibody (Internal) for IHC, ICC, IF/Immunofluorescence, WB/Western, IP LS-C352100 has an LSBio Rating of
(4.0) Rating
Publications (4)

Publications (1)

The Prostate Cancer Therapy Enzalutamide Compared with Abiraterone Acetate/Prednisone Impacts Motivation for Exploration, Spatial Learning and Alters Dopaminergic Transmission in Aged Castrated Mice. Celeste Nicola , Martine Dubois , Cynthia Campart , Tareq Al Sagheer , Laurence Desrues , Damien Schapman , Ludovic Galas , Marie Lange , Florence Joly , Hélène Castel. Cancers. 2021 Jul;13:3518.
Full Text Article PubMed: 34298734 PMC: PMC8304001
Submit a Publication for LS-C352100

Customer Reviews (0)

Submit a review for LS-C352100

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IHC‑plus™ CYP17 / CYP17A1 Antibody (aa134‑144) LS‑B8295
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
CYP17 / CYP17A1 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ CYP17 / CYP17A1 Antibody (aa358‑388) LS‑B14227
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
CYP17 / CYP17A1 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ CYP17 / CYP17A1 Antibody (aa1‑508) LS‑B13802
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
CYP17 / CYP17A1 Antibody - 1:50 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CYP17 / CYP17A1 Antibody LS‑C801327
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
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CYP17 / CYP17A1 ELISA Kit
Mouse CYP17 / CYP17A1 (Sandwich ELISA) ELISA Kit - LS-F25343
Reactivity: Mouse
Range: 1.56-100 ng/ml
See all CYP17 / CYP17A1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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