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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C22525-100 100 µg $635 
Antibody

Cyclin D1+D2 Antibody LS‑C22525

Cyclin D1+D2 Antibody LS‑C22525

Antibody:
Mouse Monoclonal to Human Cyclin D1+D2
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Mouse, Rat, Hamster
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$635 
LS-C22525-100
100 µg
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human Cyclin D1+D2
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Mouse, Rat, Hamster
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Cyclin D1+D2 antibody LS-C22525 is an unconjugated mouse monoclonal antibody to Cyclin D1+D2 from human, mouse, rat and other species. Validated for IHC, IP and WB.
Target
Human Cyclin D1+D2
Host
Mouse
Reactivity
Human, Mouse, Rat, Hamster (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • Immunoprecipitation
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant human Cyclin D1 expressed in E. coli.
Specificity
Recognizes p36 cyclin D1 and cross-reacts with p34 cyclin D2, which has a highly homologous epitope. No cross-reactivity with cyclin A, cyclin B or cyclin D3. Species cross-reactivity: human, mouse, rat and hamster.
Usage
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5 ug immunoprecipitates cyclin D1 and D2 from 500 ug of A431 RIPA lysate. Immunohistochemistry: Reported to detect cyclin D1 and D2 in paraffin-embedded sections using high energy antigen retrieval (10 mM citrate buffer, pH 6.0, microwaved 3-5 minutes, three times).
Presentation
PBS, pH 7.2, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 1/18/2020