Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C312824-10 10 µg $318 
LS-C312824-100 100 µg (0.5 mg/ml) $470 
CYBB / NOX2 / gp91phox Antibody - NOX2 / gp91phox antibody. IHC(P): Human Intestinal Cancer Tissue.
CYBB / NOX2 / gp91phox Antibody - Western blot analysis of NOX2 using anti-NOX2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOX2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NOX2 at approximately 65KD. The expected band size for NOX2 is at 65KD.
CYBB / NOX2 / gp91phox Antibody - WB of NOX2 / gp91phox antibody. All lanes: Anti-CYBB at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: JURKAT Whole Cell Lysate at 40ug. Lane 3: MCF-7 Whole Cell Lysate at 40ug. Lane 4: SMMC Whole Cell Lysate at 40ug. Predicted bind size: 65KD. Observed bind size: 65KD.
CYBB / NOX2 / gp91phox Antibody - NOX2 / gp91phox antibody. IHC(P): Human Intestinal Cancer Tissue.
CYBB / NOX2 / gp91phox Antibody - Western blot analysis of NOX2 using anti-NOX2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOX2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NOX2 at approximately 65KD. The expected band size for NOX2 is at 65KD.
CYBB / NOX2 / gp91phox Antibody - WB of NOX2 / gp91phox antibody. All lanes: Anti-CYBB at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: JURKAT Whole Cell Lysate at 40ug. Lane 3: MCF-7 Whole Cell Lysate at 40ug. Lane 4: SMMC Whole Cell Lysate at 40ug. Predicted bind size: 65KD. Observed bind size: 65KD.
1 of 3
2 of 3
3 of 3

Polyclonal Rabbit anti‑Human CYBB / NOX2 / gp91phox Antibody (aa442‑459, IHC, WB) LS‑C312824

Polyclonal Rabbit anti‑Human CYBB / NOX2 / gp91phox Antibody (aa442‑459, IHC, WB) LS‑C312824

Note: This antibody replaces LS-C388963
Antibody:
CYBB / NOX2 / gp91phox Rabbit anti-Human Polyclonal (aa442-459) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312824-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CYBB / NOX2 / gp91phox Rabbit anti-Human Polyclonal (aa442-459) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Gp91phox antibody LS-C312824 is an unconjugated rabbit polyclonal antibody to gp91phox (CYBB / NOX2) (aa442-459) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human CYBB / NOX2 / gp91phox
Synonyms
CYBB | Chronic granulomatous disease | CGD91-phox | Cytochrome b558 subunit beta | Cytochrome b(558) subunit beta | gp91-1 | gp91-PHOX | p22 phagocyte B-cytochrome | AMCBX2 | CGD | Cytochrome b-245 heavy chain | gp91PHOX | NADPH oxidase 2 | NOX2 | p91-PHOX
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human NOX2(442-459 aa YWLCRDTHAFEWFADLLQ), identical to the related rat and mouse sequences.
Epitope
aa442-459
Specificity
Detected in neutrophils (at protein level).
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CYBB / NOX2 / gp91phox
P04839 NM_000397 NP_000388.2

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024