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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CYB5A / Cytochrome b5 Antibody (clone 1A8) IHC‑plus™ LS‑B6526 Best Seller
Note: This antibody replaces LS-C133874
Cytochrome b5 antibody LS-B6526 is an unconjugated mouse monoclonal antibody to human Cytochrome b5 (CYB5A). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B6526-50
50 µg (0.49 mg/ml)
$485

Popular CYB5A / Cytochrome b5 Products

Anti-CYB5A / Cytochrome b5 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6525 concentration 5 ug/ml.
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Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

Cytochrome b5 antibody LS-B6526 is an unconjugated mouse monoclonal antibody to human Cytochrome b5 (CYB5A). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
About CYB5A / Cytochrome b5
CYB5A / Cytochrome b5 is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. P00167 NM_001914 NP_001905.1

CYB5A Antibody, CYB5 Antibody, Cytochrome b5 (microsomal) Antibody, MCB5 Antibody, Type 1 cyt-b5 Antibody, Cytochrome b-5 Antibody, Cytochrome b5 Antibody


Specifications

Target
Human CYB5A / Cytochrome b5
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [1A8]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CYB5A (AAH15182, 1 a.a. ~ 135 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human Cytochrome b5
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein. For IP 40 ug of antibody per 100 ul of protein A magnetic beads at 50 ug/ml.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry

Anti-CYB5A / Cytochrome b5 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6526 concentration 5 ug/ml.
Anti-CYB5A / Cytochrome b5 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6526 concentration 5 ug/ml.

Immunohistochemistry

Anti-CYB5A / Cytochrome b5 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6526 concentration 5 ug/ml.
Anti-CYB5A / Cytochrome b5 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6526 concentration 5 ug/ml.

Western blot

Western blot of CYB5A expression in transfected 293T cell line by CYB5A monoclonal antibody.
Western blot of CYB5A expression in transfected 293T cell line by CYB5A monoclonal antibody.

Immunoprecipitation

Immunoprecipitation of CYB5A transfected lysate using anti-CYB5A monoclonal antibody.
Immunoprecipitation of CYB5A transfected lysate using anti-CYB5A monoclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CYB5A is 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CYB5A is 0.1 ng/ml as a capture antibody.

Immunohistochemistry

Anti-CYB5A / Cytochrome b5 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6526 concentration 5 ug/ml.
Anti-CYB5A / Cytochrome b5 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6526 concentration 5 ug/ml.

Immunohistochemistry

Anti-CYB5A / Cytochrome b5 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6526 concentration 5 ug/ml.
Anti-CYB5A / Cytochrome b5 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6526 concentration 5 ug/ml.

Western blot

Western blot of CYB5A expression in transfected 293T cell line by CYB5A monoclonal antibody.
Western blot of CYB5A expression in transfected 293T cell line by CYB5A monoclonal antibody.

Immunoprecipitation

Immunoprecipitation of CYB5A transfected lysate using anti-CYB5A monoclonal antibody.
Immunoprecipitation of CYB5A transfected lysate using anti-CYB5A monoclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CYB5A is 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CYB5A is 0.1 ng/ml as a capture antibody.

Immunohistochemistry

Anti-CYB5A / Cytochrome b5 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6526 concentration 5 ug/ml.
Anti-CYB5A / Cytochrome b5 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6526 concentration 5 ug/ml.

Immunohistochemistry

Anti-CYB5A / Cytochrome b5 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6526 concentration 5 ug/ml.
Anti-CYB5A / Cytochrome b5 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6526 concentration 5 ug/ml.

Western blot

Western blot of CYB5A expression in transfected 293T cell line by CYB5A monoclonal antibody.
Western blot of CYB5A expression in transfected 293T cell line by CYB5A monoclonal antibody.

Immunoprecipitation

Immunoprecipitation of CYB5A transfected lysate using anti-CYB5A monoclonal antibody.
Immunoprecipitation of CYB5A transfected lysate using anti-CYB5A monoclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CYB5A is 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CYB5A is 0.1 ng/ml as a capture antibody.

Immunohistochemistry

Anti-CYB5A / Cytochrome b5 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6526 concentration 5 ug/ml.
Anti-CYB5A / Cytochrome b5 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6526 concentration 5 ug/ml.

Immunohistochemistry

Anti-CYB5A / Cytochrome b5 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6526 concentration 5 ug/ml.
Anti-CYB5A / Cytochrome b5 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6526 concentration 5 ug/ml.

Western blot

Western blot of CYB5A expression in transfected 293T cell line by CYB5A monoclonal antibody.
Western blot of CYB5A expression in transfected 293T cell line by CYB5A monoclonal antibody.

Immunoprecipitation

Immunoprecipitation of CYB5A transfected lysate using anti-CYB5A monoclonal antibody.
Immunoprecipitation of CYB5A transfected lysate using anti-CYB5A monoclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CYB5A is 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CYB5A is 0.1 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 9/22/2018

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