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Catalog Number Size Price
LS-C154309-1 1 mg (1 mg/ml) $722 
Cyanine Antibody - Dot Blot of Sheep anti-Cyanine Antibody Alkaline Phosphatase Conjugated. Antigen: Row 1 - Cy3 Streptavidin Conjugated Row 2 - Cy5 Streptavidin Conjugated. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: none. Secondary antibody: Sheep anti-Cyanine Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT.

Polyclonal Sheep Cyanine Antibody (AP, IHC, WB) LS‑C154309

Polyclonal Sheep Cyanine Antibody (AP, IHC, WB) LS‑C154309

Antibody:
Cyanine Sheep Polyclonal (AP) Antibody
Application:
IHC, WB, ELISA, DB
Format:
Alkaline Phosphatase, Unmodified
Price
Catalog Number
$722
LS-C154309-1
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Cyanine Sheep Polyclonal (AP) Antibody
Application:
IHC, WB, ELISA, DB
Format:
Alkaline Phosphatase, Unmodified

Specifications

Description
Cyanine antibody LS-C154309 is an AP-conjugated sheep polyclonal antibody to Cyanine. Validated for DB, ELISA, IHC and WB.
Host
Sheep
Clonality
IgG Polyclonal
Conjugations
Alkaline Phosphatase
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
A mixture of Cy3 and Cy5 conjugated KLH.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Sheep Serum and cyanine conjugated IgG. Reactivity was observed against Cy2, Cy3 and Cy5. Reactivity against other cyanine analogs is unknown.
Applications
  • IHC (1:200 - 1:1000)
  • Western blot (1:500 - 1:2500)
  • ELISA (1:2000 - 1:10000)
  • Dot Blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This product has been assayed against 1.0 ug of Cyanine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:700 to 1:2800 of the reconstitution concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.01% Sodium Azide, 50% (v/v) Glycerol
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024