Suitable for use in Neutralization, Flow Cytometry and Immunohistochemistry. Neutralization (ND50): The exact concentration required to neutralize human cell surface CXCR3 activity is dependent on the ligand concentration as well as the number of CXCR3 receptors present on the cell surface. The Neutralization Dose50 (ND50) is defined as the concentration of antibody required to yield one half maximal inhibition of the cell surface CXCR3 mediated rhl-TAC response on a responsive cell line, at a specific rhl-TAC concentration. The ND50 for this lot was determined to be ~1-6 ug/ml in the presence of 7 ng/ml of rhl-TAC, using the hCXCR3 transfected BaF/3 cells in a chemotaxis assay. Flow Cytometry: 5-10 ug/ml; Add 10 ul to 1-2.5x10^5 cells in total reaction volume 200ul. Visualize binding by adding 10 ul of 25 ug/ml IgG (FITC) goat anti-mouse. Immunohistochemistry: 10 ug/ml detected CXCR3 in human tonsil. Cells were fixed in PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. Cells were washed with PBS, BSA.
Lyophilized from PBS, pH 6.8, 5% Trehalose
Reconstitute in 200 ul sterile PBS
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only. Intended for use by laboratory professionals.
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