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Catalog Number Size Price
LS-C6283-100 100 µg $814 
Antibody

Monoclonal Mouse anti‑Human CXCR3 Antibody (clone 2AR1, IHC) LS‑C6283

Monoclonal Mouse anti‑Human CXCR3 Antibody (clone 2AR1, IHC) LS‑C6283

Antibody:
CXCR3 Mouse anti-Human Monoclonal (2AR1) Antibody
Application:
IHC, Flo, Func, Neut
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$814
LS-C6283-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CXCR3 Mouse anti-Human Monoclonal (2AR1) Antibody
Application:
IHC, Flo, Func, Neut
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
CXCR3 antibody LS-C6283 is an unconjugated mouse monoclonal antibody to human CXCR3. Validated for Flow, Func, IHC and Neut. Cited in 2 publications.
Target
Human CXCR3
Synonyms
CXCR3 | C-x-c chemokine receptor 3 | CD183 antigen | CKR-L2 | CXCR-3 | CMKAR3 | CD182 | CD183 | Cxcr3a | GPR9 | IP-10 receptor | IP10-R | Mig receptor | MigR | Mig-R | Chemokine (C-X-C) receptor 3 | CXC-R3 | Cxcr3b | G protein-coupled receptor 9 | IP10 receptor
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
2AR1
Conjugations
Unconjugated. Also available conjugated with CF, PE.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
CXCR3 transfected NS0 cells.
Specificity
Recognizes human CXCR3. Reacts specifically with CXCR3 transfectants and not the parental cell line using FACS analysis. Does not cross-react with CXCR1, CXCR2, CXCR4 or transfectants.
Applications
  • IHC (10 µg/ml)
  • Flow Cytometry (5 - 10 µg/ml)
  • Functional Assay
  • Neutralization
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for use in Neutralization, Flow Cytometry and Immunohistochemistry. Neutralization (ND50): The exact concentration required to neutralize human cell surface CXCR3 activity is dependent on the ligand concentration as well as the number of CXCR3 receptors present on the cell surface. The Neutralization Dose50 (ND50) is defined as the concentration of antibody required to yield one half maximal inhibition of the cell surface CXCR3 mediated rhl-TAC response on a responsive cell line, at a specific rhl-TAC concentration. The ND50 for this lot was determined to be ~1-6 ug/ml in the presence of 7 ng/ml of rhl-TAC, using the hCXCR3 transfected BaF/3 cells in a chemotaxis assay. Flow Cytometry: 5-10 ug/ml; Add 10 ul to 1-2.5x10^5 cells in total reaction volume 200ul. Visualize binding by adding 10 ul of 25 ug/ml IgG (FITC) goat anti-mouse. Immunohistochemistry: 10 ug/ml detected CXCR3 in human tonsil. Cells were fixed in PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. Cells were washed with PBS, BSA.
Presentation
Lyophilized from PBS, pH 6.8, 5% Trehalose
Reconstitution
Reconstitute in 200 ul sterile PBS
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CXCR3
P49682 NM_001504 NP_001495.1

LSBio Ratings

CXCR3 Antibody (clone 2AR1) for IHC, Flow LS-C6283 has an LSBio Rating of
Publications (4.1)

Publications (2)

Tumor-associated lymphocytes as an independent predictor of response to neoadjuvant chemotherapy in breast cancer. Denkert C, Loibl S, Noske A, Roller M, Mller BM, Komor M, Budczies J, Darb-Esfahani S, Kronenwett R, Hanusch C, von Trne C, Weichert W, Engels K, Solbach C, Schrader I, Dietel M, von Minckwitz G. Journal of clinical oncology : official journal of the American Society of Clinical Oncology. 2010 28:105-13. (IHC-P; Human)

Non-redundant requirement for CXCR3 signalling during tumoricidal T-cell trafficking across tumour vascular checkpoints. Mikucki ME, Fisher DT, Matsuzaki J, Skitzki JJ, Gaulin NB, Muhitch JB, Ku AW, Frelinger JG, Odunsi K, Gajewski TF, Luster AD, Evans SS. Nature communications. 2015 6:7458. (Block; Human)

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Requested From: United States
Date Requested: 8/15/2022