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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CXCL9 / MIG Antibody LS‑C104820 Best Seller
Note: This antibody replaces LS-C54206, LS-C16566
MIG antibody LS-C104820 is an unconjugated rabbit polyclonal antibody to human MIG (CXCL9). Validated for ELISA and WB.
Catalog
Size
Price
LS-C104820-50
50 µg
$285
LS-C104820-100
100 µg
$325

Popular CXCL9 / MIG Products

Biotinylated Anti-Human MIG (CXCL9) Western Blot Unreduced
Species: Human
Applications: Western blot, ELISA
MIG / CXCL9 antibody. IHC(P): Human Lung Cancer Tissue.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: Western blot, ELISA, Neutralization
Immunohistochemistry of formalin-fixed, paraffin-embedded human renal tumor with parenchymal tissue stained with Rabbit anti-Human CXCL9 following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA, Functional Assay

Product Description

MIG antibody LS-C104820 is an unconjugated rabbit polyclonal antibody to human MIG (CXCL9). Validated for ELISA and WB.

Specifications

Target
Human CXCL9 / MIG
Synonyms
CXCL9, Crg-10, Humig, SCYB9, Small-inducible cytokine B9, C-X-C motif chemokine 9, CMK, MIG
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
CXCL9 / MIG antibody was raised against e.coli derived recombinant Human MIG.
Specificity
Human MIG
Usage
Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human renal tumor with parenchyma tissue. The recommended concentration is 0.25ug/mL-0.50ug/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a pH 6 sodium citrate buffer. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hMIG (100 ng/ml), a concentration of 5-7 ug/ml of this antibody is required. ELISA: To detect hMIG by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIG (LS-C104825) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIG. Western Blot: To detect hMIG by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIG is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About CXCL9 / MIG
Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3. Q07325 NM_002416 NP_002407.1


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Images

Western blot

Anti-Human MIG (CXCL9) Western Blot Reduced
Anti-Human MIG (CXCL9) Western Blot Reduced

Western blot

Western Blot (non-reducing) of MIG / CXCL9 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of MIG / CXCL9 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Anti-Human MIG (CXCL9) Western Blot Unreduced
Anti-Human MIG (CXCL9) Western Blot Unreduced

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of MIG / CXCL9 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of MIG / CXCL9 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Anti-Human MIG (CXCL9) Sandwich ELISA
Anti-Human MIG (CXCL9) Sandwich ELISA

Western blot

Anti-Human MIG (CXCL9) Western Blot Reduced
Anti-Human MIG (CXCL9) Western Blot Reduced

Western blot

Western Blot (non-reducing) of MIG / CXCL9 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of MIG / CXCL9 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Anti-Human MIG (CXCL9) Western Blot Unreduced
Anti-Human MIG (CXCL9) Western Blot Unreduced

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of MIG / CXCL9 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of MIG / CXCL9 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Anti-Human MIG (CXCL9) Sandwich ELISA
Anti-Human MIG (CXCL9) Sandwich ELISA

Western blot

Anti-Human MIG (CXCL9) Western Blot Reduced
Anti-Human MIG (CXCL9) Western Blot Reduced

Western blot

Western Blot (non-reducing) of MIG / CXCL9 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of MIG / CXCL9 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Anti-Human MIG (CXCL9) Western Blot Unreduced
Anti-Human MIG (CXCL9) Western Blot Unreduced

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of MIG / CXCL9 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of MIG / CXCL9 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Anti-Human MIG (CXCL9) Sandwich ELISA
Anti-Human MIG (CXCL9) Sandwich ELISA

Western blot

Anti-Human MIG (CXCL9) Western Blot Reduced
Anti-Human MIG (CXCL9) Western Blot Reduced

Western blot

Western Blot (non-reducing) of MIG / CXCL9 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of MIG / CXCL9 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Anti-Human MIG (CXCL9) Western Blot Unreduced
Anti-Human MIG (CXCL9) Western Blot Unreduced

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of MIG / CXCL9 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of MIG / CXCL9 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Anti-Human MIG (CXCL9) Sandwich ELISA
Anti-Human MIG (CXCL9) Sandwich ELISA

Requested From: United States
Date Requested: 11/13/2018

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