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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CXCL10 / IP‑10 Antibody LS‑C104402 Best Seller
Note: This antibody replaces LS-C54154, LS-C16064
IP-10 antibody LS-C104402 is an unconjugated rabbit polyclonal antibody to mouse IP-10 (CXCL10). Validated for ELISA and WB.
Catalog
Size
Price
LS-C104402-50
50 µg
$285
LS-C104402-100
100 µg
$325

Popular CXCL10 / IP-10 Products

Anti-Human IP-10 (CXCL10) Western Blot Unreduced
Species: Human
Applications: Western blot, ELISA
Anti-Rat IP-10 (CXCL10) Western Blot Unreduced
Species: Rat
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

IP-10 antibody LS-C104402 is an unconjugated rabbit polyclonal antibody to mouse IP-10 (CXCL10). Validated for ELISA and WB.
About CXCL10 / IP-10
This antimicrobial gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. P02778 NM_001565 NP_001556.2

CXCL10 Antibody, C-X-C motif chemokine 10 Antibody, Gamma-IP10 Antibody, IP-10 Antibody, Gamma IP10 Antibody, GIP-10 Antibody, Mob-1 Antibody, Small-inducible cytokine B10 Antibody, Crg-2 Antibody, IFI10 Antibody, INP10 Antibody, SCYB10 Antibody


Specifications

Target
Mouse CXCL10 / IP-10
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
CXCL10 / IP-10 antibody was raised against e.coli derived recombinant Murine IP-0.
Specificity
Mouse IP-10
Usage
Immunohistochemistry: This antibody stained colchicine injected mouse brain (including the hippocampus region) tissue. The primary antibody was incubated at 1 mg/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of mIP-10 (100 ng/ml), a concentration of 10 ug/ml of this antibody is required. ELISA: To detect mIP-10 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IP-10 (LS-C104391) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIP-10. Western Blot: To detect mIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIP-10 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
sterile ddH20
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.

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Images

Western blot

Anti-Murine IP-10 (CXCL10) Western Blot Unreduced
Anti-Murine IP-10 (CXCL10) Western Blot Unreduced

Western blot

Anti-Murine IP-10 (CXCL10) Western Blot Reduced
Anti-Murine IP-10 (CXCL10) Western Blot Reduced

Western blot

Western Blot (non-reducing) of IP-10 / CXCL10 antibody LS-C104402.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IP-10 / CXCL10 antibody LS-C104402. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of IP-10 / CXCL10 antibody LS-C104402.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IP-10 / CXCL10 antibody LS-C104402. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Anti-Murine IP-10 (CXCL10) Sandwich ELISA
Anti-Murine IP-10 (CXCL10) Sandwich ELISA

Western blot

Anti-Murine IP-10 (CXCL10) Western Blot Unreduced
Anti-Murine IP-10 (CXCL10) Western Blot Unreduced

Western blot

Anti-Murine IP-10 (CXCL10) Western Blot Reduced
Anti-Murine IP-10 (CXCL10) Western Blot Reduced

Western blot

Western Blot (non-reducing) of IP-10 / CXCL10 antibody LS-C104402.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IP-10 / CXCL10 antibody LS-C104402. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of IP-10 / CXCL10 antibody LS-C104402.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IP-10 / CXCL10 antibody LS-C104402. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Anti-Murine IP-10 (CXCL10) Sandwich ELISA
Anti-Murine IP-10 (CXCL10) Sandwich ELISA

Western blot

Anti-Murine IP-10 (CXCL10) Western Blot Unreduced
Anti-Murine IP-10 (CXCL10) Western Blot Unreduced

Western blot

Anti-Murine IP-10 (CXCL10) Western Blot Reduced
Anti-Murine IP-10 (CXCL10) Western Blot Reduced

Western blot

Western Blot (non-reducing) of IP-10 / CXCL10 antibody LS-C104402.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IP-10 / CXCL10 antibody LS-C104402. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of IP-10 / CXCL10 antibody LS-C104402.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IP-10 / CXCL10 antibody LS-C104402. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Anti-Murine IP-10 (CXCL10) Sandwich ELISA
Anti-Murine IP-10 (CXCL10) Sandwich ELISA

Western blot

Anti-Murine IP-10 (CXCL10) Western Blot Unreduced
Anti-Murine IP-10 (CXCL10) Western Blot Unreduced

Western blot

Anti-Murine IP-10 (CXCL10) Western Blot Reduced
Anti-Murine IP-10 (CXCL10) Western Blot Reduced

Western blot

Western Blot (non-reducing) of IP-10 / CXCL10 antibody LS-C104402.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IP-10 / CXCL10 antibody LS-C104402. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of IP-10 / CXCL10 antibody LS-C104402.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IP-10 / CXCL10 antibody LS-C104402. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Anti-Murine IP-10 (CXCL10) Sandwich ELISA
Anti-Murine IP-10 (CXCL10) Sandwich ELISA

Requested From: United States
Date Requested: 9/23/2018

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