Immunohistochemistry: This antibody stained colchicine injected mouse brain (including the hippocampus region) tissue. The primary antibody was incubated at 1 mg/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of mIP-10 (100 ng/ml), a concentration of 10 ug/ml of this antibody is required. ELISA: To detect mIP-10 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IP-10 (LS-C104391
) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIP-10. Western Blot: To detect mIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIP-10 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.