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Antibodies > CXADR > LS-C313489
 
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Catalog Number Size Price
LS-C313489-10 10 µg $318 
LS-C313489-100 100 µg $470 
CXADR Antibody - Western blot analysis of Coxsackie Adenovirus Receptor using anti-Coxsackie Adenovirus Receptor antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysate, Lane 2: rat heart tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Coxsackie Adenovirus Receptor antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Coxsackie Adenovirus Receptor at approximately 50KD. The expected band size for Coxsackie Adenovirus Receptor is at 40KD.
CXADR Antibody - Western blot analysis of Coxsackie Adenovirus Receptor using anti-Coxsackie Adenovirus Receptor antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human U-937whole cell lysates(negative), Lane 6: human THP-1whole cell lysates(negative). After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Coxsackie Adenovirus Receptor antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Coxsackie Adenovirus Receptor at approximately 50KD. The expected band size for Coxsackie Adenovirus Receptor is at 40KD.
CXADR Antibody - WB of CXADR antibody. Lane 1: Rat Pancreas Tissue Lysate. Lane 2: Rat Brain Tissue Lysate. Lane 3: Rat Heart Tissue Lysate. Lane 4: HELA Cell Lysate. Lane 5: 293T Cell Lysate. Lane 6: COLO320 Cell Lysate.
CXADR Antibody - Western blot analysis of Coxsackie Adenovirus Receptor using anti-Coxsackie Adenovirus Receptor antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysate, Lane 2: rat heart tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Coxsackie Adenovirus Receptor antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Coxsackie Adenovirus Receptor at approximately 50KD. The expected band size for Coxsackie Adenovirus Receptor is at 40KD.
CXADR Antibody - Western blot analysis of Coxsackie Adenovirus Receptor using anti-Coxsackie Adenovirus Receptor antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human U-937whole cell lysates(negative), Lane 6: human THP-1whole cell lysates(negative). After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Coxsackie Adenovirus Receptor antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Coxsackie Adenovirus Receptor at approximately 50KD. The expected band size for Coxsackie Adenovirus Receptor is at 40KD.
CXADR Antibody - WB of CXADR antibody. Lane 1: Rat Pancreas Tissue Lysate. Lane 2: Rat Brain Tissue Lysate. Lane 3: Rat Heart Tissue Lysate. Lane 4: HELA Cell Lysate. Lane 5: 293T Cell Lysate. Lane 6: COLO320 Cell Lysate.
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Polyclonal Rabbit anti‑Human CXADR Antibody (aa313‑328, WB) LS‑C313489

Polyclonal Rabbit anti‑Human CXADR Antibody (aa313‑328, WB) LS‑C313489

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Note: This antibody replaces LS-C388129
Antibody:
CXADR Rabbit anti-Human Polyclonal (aa313-328) Antibody
Application:
WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313489-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CXADR Rabbit anti-Human Polyclonal (aa313-328) Antibody
Application:
WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CXADR antibody LS-C313489 is an unconjugated rabbit polyclonal antibody to CXADR (aa313-328) from human. It is reactive with human, mouse and rat. Validated for WB.
Target
Human CXADR
Synonyms
CXADR | CAR4/6 | Coxsackie-adenovirus receptor | CVB3-binding protein | CAR | HCAR | HCVADR
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human Coxsackie Adenovirus Receptor(313-328 aa YSKTQYNQVPSEDFER), identical to the related rat and mouse sequences.
Epitope
aa313-328
Specificity
Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitously expressed. Isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy. .
Applications
  • Western blot (0.1 - 0.5 µg/ml)
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CXADR
P78310 NM_001338 NP_001329.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
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