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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CX3CR1 Antibody LS‑C325

Catalog Number Size Price
LS-C325-0.1 0.1 mg (1 mg/ml) $365 
Immunohistochemistry of CX3CR1 in human spleen cells with CX3CR1 antibody at 10 µg/ml.
Immunofluorescence of CX3CR1 in THP1 cells with CX3CR1 antibody at 20 ug/ml.
Western blot of CX3CR1 in THP-1 cell lysate in the absence (A) or presence (B) of blocking peptide with CX3CR1 antibody at 1 ug/ml.
Immunohistochemistry of CX3CR1 in human spleen cells with CX3CR1 antibody at 10 µg/ml.
Immunofluorescence of CX3CR1 in THP1 cells with CX3CR1 antibody at 20 ug/ml.
Western blot of CX3CR1 in THP-1 cell lysate in the absence (A) or presence (B) of blocking peptide with CX3CR1 antibody at 1 ug/ml.
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CX3CR1 Antibody LS‑C325

Antibody:
Rabbit Polyclonal to Human CX3CR1
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$365 
LS-C325-0.1
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human CX3CR1
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CX3CR1 antibody LS-C325 is an unconjugated rabbit polyclonal antibody to human CX3CR1. Validated for ELISA, IF, IHC and WB.
Target
Human CX3CR1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Immunofluorescence
  • Western blot (1:500)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
a peptide corresponding to amino acids in a extracellular loop of human CX3CR1. The immunogen is located within amino acids 160 - 210 of CX3CR1.
Blocking Peptide
LS-E29911 - Liquid - 0.05 mg - $145.00
Immunizing peptide used to generate LS-C325. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Specificity
At least two isoforms of CX3CR1 are known to exist.
Usage
CX3CR1 antibody can be used for detection of CX3CR1 by Western blot at 0.5 ug/mL. Antibody can also be used for immunohistochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 39, 43 kDa Observed: 50 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CX3CR1
P49238 NM_001337 NP_001328.1

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Anti-CX3CR1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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Staining of normal human peripheral blood cells with 0.125 ug Biotin Rat IgG1 isotype control (left) or 0.125 ug Biotin anti-human CX3CR1 (right) followed by PE-streptavidin. Samples were subsequently stained intracellularly with FITC anti-human Perforin (dG9). Cells were gated on CD16+CD56+ using eFluor 450 anti-human CD16 and APC anti-human CD56 (LS-C107245). This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Requested From: United States
Date Requested: 8/20/2019
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