Cathepsin L antibody LS-B3637 is an unconjugated rabbit polyclonal antibody to Cathepsin L (CTSL) from human, mouse and rat. Validated for IHC and WB.
Rat CTSL / Cathepsin L
Rat, Human, Mouse
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (5 µg/ml)
- Western blot (0.5 - 4 µg/ml)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Synthetic peptide surrounding amino acid 223 of rat Cathepsin L.
The antibody recognizes the mature form of Cathepsin L (25 kD) from human, mouse and rat origins. Reactivity to other species has not been tested. Rat kidney tissue lysate can be used as a positive control.
Immunohistochemistry: LS-B3637 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3637 was determined to be 5 ug/ml.
PBS, 0.02% Thimerosal, 30% Glycerol, 1% BSA
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.