Cathepsin K antibody LS-B2512 is an unconjugated rabbit polyclonal antibody to Cathepsin K (CTSK) from human, mouse, rat and other species. Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Rat CTSK / Cathepsin K
Rat, Human, Mouse, Pig, Rabbit, Chicken
(tested or 100% immunogen sequence identity)
Protein A purified
- IHC - Paraffin (2.5 µg/ml)
- Western blot (0.5 - 4 µg/ml)
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Synthetic peptide surrounding amino acid 321 of rat cathepsin K.
The antibody detects proform (43 kD) and the mature form (29 kD) of cathepsin K.
Immunohistochemistry: LS-B2512 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2512 was determined to be 2.5 ug/ml.
PBS, pH 7.2, 0.5% BSA, 30% glycerol, 5 mM EDTA, 0.03% proclin
Short term: -20°C; Long term: -70°C.
For research use only.