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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CTSK / Cathepsin K Antibody (aa97‑126) LS‑C167143
Cathepsin K antibody LS-C167143 is an unconjugated rabbit polyclonal antibody to human Cathepsin K (CTSK). Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C167143-400
400 µl
$305

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Applications: IHC, Western blot, ELISA

Product Description

CTSK / Cathepsin K Antibody (aa97-126) for IHC, WB/Western, Flow LS-C167143

Specifications

Target
Human CTSK / Cathepsin K
Synonyms
CTSK, Cathepsin K, Cathepsin K (pycnodysostosis), Cathepsin O1, Cathepsin O2, Cathepsin X, CTSO2, PKND, Procathepsin K, PYCD, Cathepsin O, CTS02, CTSO1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E2021 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C167143. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa97-126
Specificity
This CTSK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97-126 amino acids from the Central region of human CTSK.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
About CTSK / Cathepsin K
CTSK / Cathepsin K is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. P43235 NM_000396 NP_000387.1


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Images

Immunohistochemistry

CTSK Antibody (Center E112) immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.
CTSK Antibody (Center E112) immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of CTSK (arrow) using rabbit polyclonal CTSK Antibody (Center E112). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CTSK gene.
Western blot of CTSK (arrow) using rabbit polyclonal CTSK Antibody (Center E112). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CTSK gene.

Flow Cytometry

CTSK Antibody (Center E112) flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CTSK Antibody (Center E112) flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

CTSK Antibody (Center E112) immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.
CTSK Antibody (Center E112) immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of CTSK (arrow) using rabbit polyclonal CTSK Antibody (Center E112). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CTSK gene.
Western blot of CTSK (arrow) using rabbit polyclonal CTSK Antibody (Center E112). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CTSK gene.

Flow Cytometry

CTSK Antibody (Center E112) flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CTSK Antibody (Center E112) flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

CTSK Antibody (Center E112) immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.
CTSK Antibody (Center E112) immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of CTSK (arrow) using rabbit polyclonal CTSK Antibody (Center E112). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CTSK gene.
Western blot of CTSK (arrow) using rabbit polyclonal CTSK Antibody (Center E112). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CTSK gene.

Flow Cytometry

CTSK Antibody (Center E112) flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CTSK Antibody (Center E112) flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

CTSK Antibody (Center E112) immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.
CTSK Antibody (Center E112) immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of CTSK (arrow) using rabbit polyclonal CTSK Antibody (Center E112). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CTSK gene.
Western blot of CTSK (arrow) using rabbit polyclonal CTSK Antibody (Center E112). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CTSK gene.

Flow Cytometry

CTSK Antibody (Center E112) flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CTSK Antibody (Center E112) flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 10/17/2018

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