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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CTSE / Cathepsin E Antibody (clone 2D5) LS‑C197003
Cathepsin E antibody LS-C197003 is an unconjugated mouse monoclonal antibody to human Cathepsin E (CTSE). Validated for ELISA and WB.
Catalog
Size
Price
LS-C197003-100
100 µg
$355

Popular CTSE / Cathepsin E Products

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Product Description

CTSE / Cathepsin E Antibody (clone 2D5) for WB/Western, ELISA LS-C197003

Specifications

Target
Human CTSE / Cathepsin E
Synonyms
CTSE, CATE, Cathepsin E, Ctsea, Slow-moving proteinase, CEB
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [2D5]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
CTSE (AAH42537, aa18-396 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human CTSE / Cathepsin E
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
About CTSE / Cathepsin E
CTSE / Cathepsin E is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. P14091 AJ250717 AAA52300.1


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Images

Western blot

Western Blot analysis of CTSE expression in transfected 293T cell line by CTSE monoclonal antibody (M10), clone 2D5.Lane 1: CTSE transfected lysate (Predicted MW: 42.8 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CTSE expression in transfected 293T cell line by CTSE monoclonal antibody (M10), clone 2D5.Lane 1: CTSE transfected lysate (Predicted MW: 42.8 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CTSE is 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CTSE is 1 ng/ml as a capture antibody.

Western blot

Western Blot analysis of CTSE expression in transfected 293T cell line by CTSE monoclonal antibody (M10), clone 2D5.Lane 1: CTSE transfected lysate (Predicted MW: 42.8 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CTSE expression in transfected 293T cell line by CTSE monoclonal antibody (M10), clone 2D5.Lane 1: CTSE transfected lysate (Predicted MW: 42.8 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CTSE is 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CTSE is 1 ng/ml as a capture antibody.

Western blot

Western Blot analysis of CTSE expression in transfected 293T cell line by CTSE monoclonal antibody (M10), clone 2D5.Lane 1: CTSE transfected lysate (Predicted MW: 42.8 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CTSE expression in transfected 293T cell line by CTSE monoclonal antibody (M10), clone 2D5.Lane 1: CTSE transfected lysate (Predicted MW: 42.8 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CTSE is 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CTSE is 1 ng/ml as a capture antibody.

Western blot

Western Blot analysis of CTSE expression in transfected 293T cell line by CTSE monoclonal antibody (M10), clone 2D5.Lane 1: CTSE transfected lysate (Predicted MW: 42.8 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CTSE expression in transfected 293T cell line by CTSE monoclonal antibody (M10), clone 2D5.Lane 1: CTSE transfected lysate (Predicted MW: 42.8 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CTSE is 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CTSE is 1 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 10/22/2018

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