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Catalog Number Size Price
LS-C745354-25 25 µl (1.15 mg/ml) $275 
CTNNB1 / Beta Catenin Antibody - Immunohistochemistry of rabbit anti-beta Catenin antibody. Tissue: human small intestine epithelium. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: beta Catenin antibody at 5-10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: Strong membranous staining in a variety of epithelial tissues. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
CTNNB1 / Beta Catenin Antibody - Western Blot of rabbit anti-Beta Catenin antibody. Marker: Opal Pre-stained ladder Lane 1: HEK293 lysate Lane 2: HeLa Lysate Lane 3: MCF-7 Lysate Lane 4: Jurkat Lysate Lane 5: A431 Lysate Lane 6: A549 Lysate Lane 7: LNCap Lysate Lane 8: MOLT-4 Lysate Lane 9: Ramos Lysate Lane 10: Raji Lsyate Lane 11: A-172 Lysate Lane 12: NIH/3T3 Lysate Load: 35 µg per lane. Primary antibody: Beta catenin antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 85 kDa for Beta Catenin.
CTNNB1 / Beta Catenin Antibody - Western Blot of rabbit anti-catenin ß-1 antibody Lane 1: zebrafish embryos injected with myc tagged catenin ß 1 mRNA Lane 2: zebrafish embryos injected with myc tagged catenin ß 2 mRNA Lane 3: zebrafish embryos un-injected Primary antibody: catenin ß-1 antibody at 1:500 overnight at 4°C Secondary antibody: goat anti-rabbit HRP at 1:10,000 for 1 hour at RT Predicted/Observed size: 85.5kDa/ ~125kDa (arrow) endogenous catenin ß-1 Other band(s): ~110kDa (star) co-migrating catenin ß-1 and ß-2.
CTNNB1 / Beta Catenin Antibody - Immunohistochemistry of rabbit anti-beta Catenin antibody. Tissue: human small intestine epithelium. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: beta Catenin antibody at 5-10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: Strong membranous staining in a variety of epithelial tissues. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
CTNNB1 / Beta Catenin Antibody - Western Blot of rabbit anti-Beta Catenin antibody. Marker: Opal Pre-stained ladder Lane 1: HEK293 lysate Lane 2: HeLa Lysate Lane 3: MCF-7 Lysate Lane 4: Jurkat Lysate Lane 5: A431 Lysate Lane 6: A549 Lysate Lane 7: LNCap Lysate Lane 8: MOLT-4 Lysate Lane 9: Ramos Lysate Lane 10: Raji Lsyate Lane 11: A-172 Lysate Lane 12: NIH/3T3 Lysate Load: 35 µg per lane. Primary antibody: Beta catenin antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 85 kDa for Beta Catenin.
CTNNB1 / Beta Catenin Antibody - Western Blot of rabbit anti-catenin ß-1 antibody Lane 1: zebrafish embryos injected with myc tagged catenin ß 1 mRNA Lane 2: zebrafish embryos injected with myc tagged catenin ß 2 mRNA Lane 3: zebrafish embryos un-injected Primary antibody: catenin ß-1 antibody at 1:500 overnight at 4°C Secondary antibody: goat anti-rabbit HRP at 1:10,000 for 1 hour at RT Predicted/Observed size: 85.5kDa/ ~125kDa (arrow) endogenous catenin ß-1 Other band(s): ~110kDa (star) co-migrating catenin ß-1 and ß-2.
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Polyclonal Rabbit anti‑Human CTNNB1 / Beta Catenin Antibody (C‑Terminus, IHC, WB) LS‑C745354

Polyclonal Rabbit anti‑Human CTNNB1 / Beta Catenin Antibody (C‑Terminus, IHC, WB) LS‑C745354

Antibody:
CTNNB1 / Beta Catenin Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Zebrafish, Xenopus laevis
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745354-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
CTNNB1 / Beta Catenin Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Zebrafish, Xenopus laevis
Format:
Unconjugated, Unmodified

Specifications

Description
Beta Catenin antibody LS-C745354 is an unconjugated rabbit polyclonal antibody to Beta Catenin (CTNNB1) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB.
Target
Human CTNNB1 / Beta Catenin
Synonyms
CTNNB1 | Beta-catenin | Catenin beta-1 | CTNNB | Beta Catenin
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Zebrafish, Xenopus laevis (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bovine, Zebrafish, Xenopus laevis (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Beta catenin antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to catenin beta-1 C-Terminus.
Epitope
C-Terminus
Specificity
A BLAST analysis was used to suggest cross-reactivity with catenin beta-1 protein from human, mouse, rat, bovine, Xenopus laevis, and Danio rerio at 100% based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Applications
  • IHC (5 - 10 µg/ml)
  • Western blot (1:500)
  • ELISA (1:25000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CTNNB1 / Beta Catenin
P35222 NM_001904 NP_001895.1

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Request SDS/MSDS

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Requested From: United States
Date Requested: 5/11/2021