Target
Human CTCF
Synonyms
CTCF | 11-zinc finger protein | CCCTC-binding factor | Transcriptional repressor CTCF | CTCFL paralog
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence CHLPQNQTDGGE, from the internal region of the protein sequence according to NP_006556.1 .
Applications
- Western blot (0.1 - 0.5 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:1000)
Usage
Peptide ELISA: antibody detection limit dilution 1:1000. Western blot: Approx 150kDa band observed in lysates of cell line Jurkat and nuclear Jurkat (calculated MW of 82.8kDa according to NP_006556.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee