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Catalog Number Size Price
LS-C748328-20 20 µl $275 
LS-C748328-50 50 µl $311 
LS-C748328-100 100 µl $389 
LS-C748328-200 200 µl $503 
CTCF Antibody - Immunohistochemistry of paraffin-embedded mouse heart using CTCF antibody at dilution of 1:100 (40x lens).
CTCF Antibody - Immunohistochemistry of paraffin-embedded mouse brain using CTCF antibody at dilution of 1:100 (20x lens).
CTCF Antibody - Immunohistochemistry of paraffin-embedded rat brain using CTCF antibody at dilution of 1:100 (20x lens).
CTCF Antibody - Immunofluorescence analysis of U2OS cells using CTCF antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
CTCF Antibody - Western blot analysis of extracts of various cell lines, using CTCF antibody at 1:500 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.
CTCF Antibody - Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug CTCF antibody. Western blot was performed from the immunoprecipitate using CTCF antibody at a dilition of 1:1000.
CTCF Antibody - Chromatin immunoprecipitation analysis extracts of HCT116 cells, using CTCF antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
CTCF Antibody - Immunohistochemistry of paraffin-embedded mouse heart using CTCF antibody at dilution of 1:100 (40x lens).
CTCF Antibody - Immunohistochemistry of paraffin-embedded mouse brain using CTCF antibody at dilution of 1:100 (20x lens).
CTCF Antibody - Immunohistochemistry of paraffin-embedded rat brain using CTCF antibody at dilution of 1:100 (20x lens).
CTCF Antibody - Immunofluorescence analysis of U2OS cells using CTCF antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
CTCF Antibody - Western blot analysis of extracts of various cell lines, using CTCF antibody at 1:500 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.
CTCF Antibody - Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug CTCF antibody. Western blot was performed from the immunoprecipitate using CTCF antibody at a dilition of 1:1000.
CTCF Antibody - Chromatin immunoprecipitation analysis extracts of HCT116 cells, using CTCF antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Polyclonal Rabbit anti‑Human CTCF Antibody (IHC, IF, WB) LS‑C748328

Polyclonal Rabbit anti‑Human CTCF Antibody (IHC, IF, WB) LS‑C748328

Antibody:
CTCF Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, IP, ChrIP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C748328-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CTCF Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, IP, ChrIP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CTCF antibody LS-C748328 is an unconjugated rabbit polyclonal antibody to CTCF from human. It is reactive with human, mouse and rat. Validated for ChrIP, IF, IHC, IP and WB.
Target
Human CTCF
Synonyms
CTCF | 11-zinc finger protein | CCCTC-binding factor | Transcriptional repressor CTCF | CTCFL paralog
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1). MEGDAVEAIVEESETFIKGKERKTYQRRREGGQEEDACHLPQNQTDGGEVVQDVNSSVQMVMMEQLDPTLLQMKTEVMEGTVAPEAEAAVDDTQIITLQVVNMEEQPINIGELQLVQVPVPVTVPVATTSVEELQGAYENEVSKEGLAESEPMICHTLPLPEGFQVVKVGANGEVETLEQGELPPQEDPSWQKDPDYQPPAKKTKKTKKSKLRYTEEGKDVDVSVYDFEEEQQEGLLSEVNAEKVVGNMKPPKPTKIKKK
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation (1:50 - 1:100)
  • Chromatin Immunoprecipitation (1:50 - 1:200)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 45kDa/82kDa, while the observed MW by Western blot was 135kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CTCF
P49711 NM_006565 NP_006556.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024