Products
Research Areas
COVID-19
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
206-374-1102
Fax:
206-577-4565
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, Proforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C331282-20 20 µl $263 
LS-C331282-50 50 µl $301 
LS-C331282-100 100 µl $359 
LS-C331282-200 200 µl $473 
CTCF Antibody - Immunohistochemistry of paraffin-embedded mouse heart tissue.
CTCF Antibody - Immunohistochemistry of paraffin-embedded mouse brain tissue.
CTCF Antibody - Immunohistochemistry of paraffin-embedded rat brain tissue.
CTCF Antibody - Immunofluorescence analysis of U2OS cells using CTCF antibodyat dilution of 1:100. Blue: DAPI for nuclear staining.
CTCF Antibody - Western blot analysis of extracts of various cell lines, using CTCF antibody at 1:500 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
CTCF Antibody - Chromatin immunoprecipitation analysis extracts of HCT116 cells, using CTCF antibodyand rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
CTCF Antibody - Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug CTCF antibody. Western blot was performed from the immunoprecipitate using CTCF antibodyat a dilition of 1:1000.
CTCF Antibody - Immunohistochemistry of paraffin-embedded mouse heart tissue.
CTCF Antibody - Immunohistochemistry of paraffin-embedded mouse brain tissue.
CTCF Antibody - Immunohistochemistry of paraffin-embedded rat brain tissue.
CTCF Antibody - Immunofluorescence analysis of U2OS cells using CTCF antibodyat dilution of 1:100. Blue: DAPI for nuclear staining.
CTCF Antibody - Western blot analysis of extracts of various cell lines, using CTCF antibody at 1:500 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
CTCF Antibody - Chromatin immunoprecipitation analysis extracts of HCT116 cells, using CTCF antibodyand rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
CTCF Antibody - Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug CTCF antibody. Western blot was performed from the immunoprecipitate using CTCF antibodyat a dilition of 1:1000.
1 of 7
2 of 7
3 of 7
4 of 7
5 of 7
6 of 7
7 of 7

Polyclonal Rabbit anti‑Human CTCF Antibody (IHC, IF, WB) LS‑C331282

Polyclonal Rabbit anti‑Human CTCF Antibody (IHC, IF, WB) LS‑C331282

Antibody:
CTCF Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, IP
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$263
LS-C331282-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CTCF Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, IP
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CTCF antibody LS-C331282 is an unconjugated rabbit polyclonal antibody to CTCF from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC, IP and WB.
Target
Human CTCF
Synonyms
CTCF | 11-zinc finger protein | CCCTC-binding factor | Transcriptional repressor CTCF | CTCFL paralog
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1). MEGDAVEAIVEESETFIKGKERKTYQRRREGGQEEDACHLPQNQTDGGEVVQDVNSSVQMVMMEQLDPTLLQMKTEVMEGTVAPEAEAAVDDTQIITLQVVNMEEQPINIGELQLVQVPVPVTVPVATTSVEELQGAYENEVSKEGLAESEPMICHTLPLPEGFQVVKVGANGEVETLEQGELPPQEDPSWQKDPDYQPPAKKTKKTKKSKLRYTEEGKDVDVSVYDFEEEQQEGLLSEVNAEKVVGNMKPPKPTKIKKK
Specificity
Human CTCF
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 45kDa/82kDa, while the observed MW by Western blot was 135kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CTCF
P49711 NM_006565 NP_006556.1

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/28/2022