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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CSTF3 Antibody (clone 1D4) LS‑C133142
CSTF3 antibody LS-C133142 is an unconjugated mouse monoclonal antibody to human CSTF3. Validated for ELISA, IF, IHC and WB.
Catalog
Size
Price
LS-C133142-100
100 µg (1 mg/ml)
$355

Popular CSTF3 Products

Detection of Human and Mouse CSTF77 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-CSTF77 used at a dilution of 1:200 (1 ug/ml). Detection: DAB.
Species: Human, Mouse, Rat, Bovine, Dog, Horse, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat, Bovine, Dog, Horse, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Immunofluorescence
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit, Chicken, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Detection of Human CSTF77 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CSTF77 used at a dilution of 1:200 (1 ug/ml). Detection: DAB.
Species: Human, Bovine, Horse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation

Product Description

CSTF3 antibody LS-C133142 is an unconjugated mouse monoclonal antibody to human CSTF3. Validated for ELISA, IF, IHC and WB.
About CSTF3
CSTF3 is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms. Q12996 NM_001326 NP_001317.1

CSTF3 Antibody, CSTF 77 kDa subunit Antibody, CF-1 77 kDa subunit Antibody, CSTF-77 Antibody


Specifications

Target
Human CSTF3
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [1D4]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CSTF3 antibody was raised against cSTF3 (AAH09792, 1 a.a. ~ 104 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human CSTF3
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to CSTF3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml].
Immunoperoxidase of monoclonal antibody to CSTF3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml].

Immunofluorescence

Immunofluorescence of monoclonal antibody to CSTF3 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to CSTF3 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot of CSTF3 expression in transfected 293T cell line by CSTF3 monoclonal antibody (M01), clone 1D4.
Western blot of CSTF3 expression in transfected 293T cell line by CSTF3 monoclonal antibody (M01), clone 1D4.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CSTF3 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CSTF3 is approximately 0.1 ng/ml as a capture antibody.

RNA interference

Western blot of CSTF3 over-expressed 293 cell line, cotransfected with CSTF3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CSTF3 monoclonal antibody, clone 1D4. GAPDH ( 36.1 kD ) used as specificity.
Western blot of CSTF3 over-expressed 293 cell line, cotransfected with CSTF3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CSTF3 monoclonal antibody, clone 1D4. GAPDH ( 36.1 kD ) used as specificity.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to CSTF3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml].
Immunoperoxidase of monoclonal antibody to CSTF3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml].

Immunofluorescence

Immunofluorescence of monoclonal antibody to CSTF3 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to CSTF3 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot of CSTF3 expression in transfected 293T cell line by CSTF3 monoclonal antibody (M01), clone 1D4.
Western blot of CSTF3 expression in transfected 293T cell line by CSTF3 monoclonal antibody (M01), clone 1D4.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CSTF3 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CSTF3 is approximately 0.1 ng/ml as a capture antibody.

RNA interference

Western blot of CSTF3 over-expressed 293 cell line, cotransfected with CSTF3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CSTF3 monoclonal antibody, clone 1D4. GAPDH ( 36.1 kD ) used as specificity.
Western blot of CSTF3 over-expressed 293 cell line, cotransfected with CSTF3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CSTF3 monoclonal antibody, clone 1D4. GAPDH ( 36.1 kD ) used as specificity.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to CSTF3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml].
Immunoperoxidase of monoclonal antibody to CSTF3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml].

Immunofluorescence

Immunofluorescence of monoclonal antibody to CSTF3 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to CSTF3 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot of CSTF3 expression in transfected 293T cell line by CSTF3 monoclonal antibody (M01), clone 1D4.
Western blot of CSTF3 expression in transfected 293T cell line by CSTF3 monoclonal antibody (M01), clone 1D4.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CSTF3 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CSTF3 is approximately 0.1 ng/ml as a capture antibody.

RNA interference

Western blot of CSTF3 over-expressed 293 cell line, cotransfected with CSTF3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CSTF3 monoclonal antibody, clone 1D4. GAPDH ( 36.1 kD ) used as specificity.
Western blot of CSTF3 over-expressed 293 cell line, cotransfected with CSTF3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CSTF3 monoclonal antibody, clone 1D4. GAPDH ( 36.1 kD ) used as specificity.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to CSTF3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml].
Immunoperoxidase of monoclonal antibody to CSTF3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml].

Immunofluorescence

Immunofluorescence of monoclonal antibody to CSTF3 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to CSTF3 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot of CSTF3 expression in transfected 293T cell line by CSTF3 monoclonal antibody (M01), clone 1D4.
Western blot of CSTF3 expression in transfected 293T cell line by CSTF3 monoclonal antibody (M01), clone 1D4.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CSTF3 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CSTF3 is approximately 0.1 ng/ml as a capture antibody.

RNA interference

Western blot of CSTF3 over-expressed 293 cell line, cotransfected with CSTF3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CSTF3 monoclonal antibody, clone 1D4. GAPDH ( 36.1 kD ) used as specificity.
Western blot of CSTF3 over-expressed 293 cell line, cotransfected with CSTF3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CSTF3 monoclonal antibody, clone 1D4. GAPDH ( 36.1 kD ) used as specificity.

Requested From: United States
Date Requested: 8/19/2018

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