CSRP3 antibody LS-C667885 is an unconjugated rabbit polyclonal antibody to human CSRP3. Validated for ICC, IF and WB.
(tested or 100% immunogen sequence identity)
- Immunofluorescence (1:50 - 1:200)
- Western blot (1:500 - 1:2000)
CSRP3 antibody was raised against recombinant full length protein of human CSRP3
Recognizes endogenous levels of CSRP3 protein.
Western blot analysis of CSRP3 expression in mouse skeletal muscle (A), rat heart (B) whole cell lysates. Immunofluorescent analysis of CSRP3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
For research use only.