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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CSRP3 Antibody (aa150‑163) IHC‑plus™ LS‑B6484 Best Seller
Note: This antibody replaces LS-C87391
CSRP3 antibody LS-B6484 is an unconjugated goat polyclonal antibody to CSRP3 from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B6484-50
50 µg (0.5 mg/ml)
$425

Popular CSRP3 Products

Fetal Muscle Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
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Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot

Product Description

CSRP3 antibody LS-B6484 is an unconjugated goat polyclonal antibody to CSRP3 from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About CSRP3
CSRP3 is a member of the CSRP family of LIM domain proteins, which may be involved in regulatory processes important for development and cellular differentiation. The LIM/double zinc-finger motif found in this protein is found in a group of proteins with critical functions in gene regulation, cell growth, and somatic differentiation. Mutations in this gene are thought to cause heritable forms of hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM) in humans. P50461 NM_003476 NP_003467.1

CSRP3 Antibody, Cardiac LIM domain protein Antibody, Cardiac LIM protein Antibody, CLP Antibody, CMH12 Antibody, Cysteine-rich protein 3 Antibody, CMD1M Antibody, CRP3 Antibody, Muscle LIM protein Antibody, LIM domain only 4 Antibody, LIM domain protein, cardiac Antibody, MLP Antibody


Specifications

Target
Human CSRP3
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Predicted
Zebrafish (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 - 3.75 µg/ml)
  • Western blot (0.01 - 0.03 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000) & (0.01 - 0.03 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CSRP3 antibody was raised against synthetic peptide CGKSLESTNVTDKD from an internal region of human CSRP3 (NP_003467.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Chicken, Platypus, Lizard (100%); Salmon, Stickleback, Pufferfish, Zebrafish (93%).
Blocking Peptide
LS-E26951 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B6484. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa150-163
Specificity
Human CSRP3.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx. 20kD band observed in Human, Mouse and Rat Heart lysates (calculated MW of 21.0kD according to Human NP_003467.1 and 20.8kD according to Rat NP_476485.1). Recommended concentration: 0.01-0.03 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry

Anti-CSRP3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6484 concentration 2.5 ug/ml.
Anti-CSRP3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6484 concentration 2.5 ug/ml.

Immunohistochemistry

Anti-CSRP3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6484 concentration 2.5 ug/ml.
Anti-CSRP3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6484 concentration 2.5 ug/ml.

Western blot

EB09104 (0.01µg/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09104 (0.01µg/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Antibody (0.01 ug/ml) staining of Rat Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.01 ug/ml) staining of Rat Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-CSRP3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6484 concentration 2.5 ug/ml.
Anti-CSRP3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6484 concentration 2.5 ug/ml.

Immunohistochemistry

Anti-CSRP3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6484 concentration 2.5 ug/ml.
Anti-CSRP3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6484 concentration 2.5 ug/ml.

Western blot

EB09104 (0.01µg/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09104 (0.01µg/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Antibody (0.01 ug/ml) staining of Rat Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.01 ug/ml) staining of Rat Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-CSRP3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6484 concentration 2.5 ug/ml.
Anti-CSRP3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6484 concentration 2.5 ug/ml.

Immunohistochemistry

Anti-CSRP3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6484 concentration 2.5 ug/ml.
Anti-CSRP3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6484 concentration 2.5 ug/ml.

Western blot

EB09104 (0.01µg/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09104 (0.01µg/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Antibody (0.01 ug/ml) staining of Rat Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.01 ug/ml) staining of Rat Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-CSRP3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6484 concentration 2.5 ug/ml.
Anti-CSRP3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6484 concentration 2.5 ug/ml.

Immunohistochemistry

Anti-CSRP3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6484 concentration 2.5 ug/ml.
Anti-CSRP3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6484 concentration 2.5 ug/ml.

Western blot

EB09104 (0.01µg/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09104 (0.01µg/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Antibody (0.01 ug/ml) staining of Rat Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.01 ug/ml) staining of Rat Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 9/24/2018

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