NG2 antibody LS-C22113 is an unconjugated mouse monoclonal antibody to human NG2 (CSPG4). Validated for Flow, Func, IF and IP.
(tested or 100% immunogen sequence identity)
Protein A purified
- Flow Cytometry
- Functional Assay
CSPG4 / NG2 antibody was raised against human M14 melanoma cell extract depleted of fibronectin and bound by lens culinaris lectin-Sepharose.
Recognizes mature chondroitin sulfate proteoglycan core glycoprotein of 250kD as well as precursor polypeptides of 210, 220 and 240kD. Reacts with human melanoma, glioma and proliferating brain endothelial cells.
Suitable for use in Immunofluorescence Microscopy, Immunoprecipitation, Immunoelectron microscopy, FACS analysis, and Functional Block. Immunofluorescence Microscopy: Cells in culture. Immunoprecipitation: 1 ug/1E6 cells. FACS analysis: 1-2 ug/1E6 cells. Functional Block: Melanoma cell spreading, glioma cell proliferation, and tumor growth in nude mice. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
PBS, pH 7.6. No preservative added. Glycerol free.
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.