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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C22113-100 100 µg (1 mg/ml) $860 
Antibody

Monoclonal Mouse anti‑Human CSPG4 / NG2 Antibody (Azide‑free, IF) LS‑C22113

Monoclonal Mouse anti‑Human CSPG4 / NG2 Antibody (Azide‑free, IF) LS‑C22113

Antibody:
CSPG4 / NG2 Mouse anti-Human Monoclonal (Azide-free) Antibody
Application:
IF, IP, Flo, Func
Reactivity:
Human
Format:
Unconjugated, Azide-free
Price
Catalog Number
$860
LS-C22113-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
CSPG4 / NG2 Mouse anti-Human Monoclonal (Azide-free) Antibody
Application:
IF, IP, Flo, Func
Reactivity:
Human
Format:
Unconjugated, Azide-free

Specifications

Description
NG2 antibody LS-C22113 is an unconjugated mouse monoclonal antibody to human NG2 (CSPG4). Validated for Flow, Func, IF and IP.
Target
Human CSPG4 / NG2
Synonyms
CSPG4 | MCSP | MEL-CSPG | MSK16 | NG2 | HMW-MAA | MCSPG
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Azide-free
Immunogen
Human M14 melanoma cell extract depleted of fibronectin and bound by lens culinaris lectin-Sepharose.
Specificity
Recognizes mature chondroitin sulfate proteoglycan core glycoprotein of 250kD as well as precursor polypeptides of 210, 220 and 240kD. Reacts with human melanoma, glioma and proliferating brain endothelial cells.
Applications
  • Immunofluorescence
  • Immunoprecipitation
  • Flow Cytometry
  • Functional Assay
Usage
Suitable for use in Immunofluorescence Microscopy, Immunoprecipitation, Immunoelectron microscopy, FACS analysis, and Functional Block. Immunofluorescence Microscopy: Cells in culture. Immunoprecipitation: 1 ug/1E6 cells. FACS analysis: 1-2 ug/1E6 cells. Functional Block: Melanoma cell spreading, glioma cell proliferation, and tumor growth in nude mice. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.6. No preservative added. Glycerol free.
Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CSPG4 / NG2
Q6UVK1 NM_001897 NP_001888.2

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Requested From: United States
Date Requested: 10/31/2020