G-CSF antibody LS-C15556 is a biotin-conjugated rat monoclonal antibody to human G-CSF (CSF3). Validated for ELISA and WB.
Human CSF3 / G-CSF
(tested or 100% immunogen sequence identity)
CSF3 / G-CSF antibody was raised against e. coli-expressed recombinant human G-CSF.
Recognizes human Granulocyte-Colony Stimulating Factor (G-CSF).
ELISA Detection: Useful as a detection antibody in a sandwich ELISA for quantifying human G-CSF protein levels.1,2 Biotinylated antibody should be paired with a suitable purified antibody as the capture antibody, with purified recombinant human G-CSF as the standard. Western Blot: Useful for Western blot applications. A concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human G-CSF under reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, 0.1% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.