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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CSF2 / GM‑CSF Antibody LS‑C154129

Catalog Number Size Price
LS-C154129-100 100 µg (1 mg/ml) $425 
Anti-Mouse GM-CSF Antibody - Western Blot. Western blot of anti-Mouse GM-CSF antibody shows detection of a band ~14 kD in size corresponding to recombinant mouse GM-CSF (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1000 dilution. Detection occurred using DyLight649 conjugated anti-Rabbit IgG ( secondary antibody diluted 1:20000 in blocking buffer (p/n MB-070). Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).

CSF2 / GM‑CSF Antibody LS‑C154129

Antibody:
Rabbit Polyclonal to Mouse CSF2 / GM-CSF
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C154129-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Mouse CSF2 / GM-CSF
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
GM-CSF antibody LS-C154129 is an unconjugated rabbit polyclonal antibody to mouse GM-CSF (CSF2 ). Validated for ELISA and WB.
Target
Mouse CSF2 / GM-CSF
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Purified, heated to 56°C for 30 minutes
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:2000)
  • ELISA
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse GM-CSF protein.
Specificity
This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse GM-CSF in cell supernatants and certain body fluids. This antibody is useful for neutralization of mouse GM-CSF in bioassays. For neutralization, it is recommended to incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Usage
This purified antibody has been tested for use in neutralizations, ELISA and western blotting. Reactivity is also expected in radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 14.3 kD in size corresponding to mouse GM-CSF protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
Reconstitution
Reconstitute in deionized water
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CSF2 / GM-CSF
P04141 NM_000758 NP_000749.2

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Requested From: United States
Date Requested: 8/23/2019
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