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Antibodies > CRP / C-Reactive Protein > LS-C355422
 
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Catalog Number Size Price
LS-C355422-1 1 mg $857 
CRP / C-Reactive Protein Antibody - Immunofluorescent analysis of C Reactive Protein was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with C Reactive Protein (P4D7) Mouse Monoclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
CRP / C-Reactive Protein Antibody - Western blot analysis was performed on membrane enriched extracts (30 ug) of Mouse liver (Lane 1), PANC-1 (Lane 2) and HeLa (Lane 3).The blots were probed with Anti-C Reactive Protein Mouse Monoclonal Antibody and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, HRP conjugate. A ~25 kDa band corresponding C Reactive Protein was observed across cell lines and tissues tested. Known quantity of protein samples were electrophoresed using12 % Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody using iBind Flex Western Starter Kit.
CRP / C-Reactive Protein Antibody - Immunofluorescent analysis of C Reactive Protein was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with C Reactive Protein (P4D7) Mouse Monoclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
CRP / C-Reactive Protein Antibody - Western blot analysis was performed on membrane enriched extracts (30 ug) of Mouse liver (Lane 1), PANC-1 (Lane 2) and HeLa (Lane 3).The blots were probed with Anti-C Reactive Protein Mouse Monoclonal Antibody and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, HRP conjugate. A ~25 kDa band corresponding C Reactive Protein was observed across cell lines and tissues tested. Known quantity of protein samples were electrophoresed using12 % Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody using iBind Flex Western Starter Kit.
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Monoclonal Mouse anti‑Human CRP / C‑Reactive Protein Antibody (clone P4D7, IF, WB) LS‑C355422

Monoclonal Mouse anti‑Human CRP / C‑Reactive Protein Antibody (clone P4D7, IF, WB) LS‑C355422

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Antibody:
CRP / C-Reactive Protein Mouse anti-Human Monoclonal (P4D7) Antibody
Application:
IF, WB, IP, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$857
LS-C355422-1
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CRP / C-Reactive Protein Mouse anti-Human Monoclonal (P4D7) Antibody
Application:
IF, WB, IP, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
C-Reactive Protein antibody LS-C355422 is an unconjugated mouse monoclonal antibody to human C-Reactive Protein (CRP). Validated for ELISA, IF, IP, RIA and WB.
Target
Human CRP / C-Reactive Protein
Synonyms
CRP | C-reactive protein | Pentraxin 1 | PTX1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
P4D7
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Immunogen
Purified human plasma CRP
Specificity
The antibody targets C Reactive Protein in ELISA, IP, and RIA applications and shows reactivity with Human samples. It detects C Reactive Protein which has a predicted molecular weight of approximately 23 kD.
Applications
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • ELISA
  • Radioimmunoassay
Presentation
0.015 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CRP / C-Reactive Protein
P02741 NM_000567 NP_000558.2

Publications (0)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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