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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CRIP1 Antibody (aa48‑77) IHC‑plus™ LS‑B13196
Note: This antibody replaces LS-C167041
CRIP1 antibody LS-B13196 is an unconjugated rabbit polyclonal antibody to CRIP1 from human and mouse. Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B13196-200
200 µl
$425

Popular CRIP1 Products

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Pig, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Mouse
Applications: IHC, Western blot, Flow Cytometry, ELISA
Immunohistochemistry of paraffin-embedded human kidney tissue at dilution 1:100
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Antibody
Species: Human, Mouse
Applications: IHC, Western blot, Flow Cytometry, ELISA

Product Description

CRIP1 antibody LS-B13196 is an unconjugated rabbit polyclonal antibody to CRIP1 from human and mouse. Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About CRIP1
Cysteine-rich intestinal protein (CRIP) belongs to the LIM/double zinc finger protein family, members of which include cysteine- and glycine-rich protein-1 (CSRP1; MIM 123876), rhombotin-1 (RBTN1; MIM 186921), rhombotin-2 (RBTN2; MIM 180385), and rhombotin-3 (RBTN3; MIM 180386). CRIP may be involved in intestinal zinc transport (Hempe and Cousins, 1991 [PubMed 1946385]). P50238 NM_001311 NP_001302.1

CRIP1 Antibody, CRIP Antibody, Cysteine-rich heart protein Antibody, Cysteine-rich protein 1 Antibody, CRP1 Antibody, HCRHP Antibody, CRHP Antibody, CRP-1 Antibody


Specifications

Target
Human CRIP1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Rat, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E7558 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B13196. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa48-77
Specificity
This CRIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-77 amino acids from the C-terminal region of human CRIP1.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.

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Images

Immunohistochemistry - Paraffin

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot of CRIP1 Antibody in HL-60 cell line lysates (35 ug/lane). CRIP1 (arrow) was detected using the purified antibody.
Western blot of CRIP1 Antibody in HL-60 cell line lysates (35 ug/lane). CRIP1 (arrow) was detected using the purified antibody.

Western blot

Western blot of CRIP1 Antibody in mouse bladder tissue lysates (35 ug/lane). CRIP1 (arrow) was detected using the purified antibody.
Western blot of CRIP1 Antibody in mouse bladder tissue lysates (35 ug/lane). CRIP1 (arrow) was detected using the purified antibody.

Flow Cytometry

CRIP1 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CRIP1 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry - Paraffin

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot of CRIP1 Antibody in HL-60 cell line lysates (35 ug/lane). CRIP1 (arrow) was detected using the purified antibody.
Western blot of CRIP1 Antibody in HL-60 cell line lysates (35 ug/lane). CRIP1 (arrow) was detected using the purified antibody.

Western blot

Western blot of CRIP1 Antibody in mouse bladder tissue lysates (35 ug/lane). CRIP1 (arrow) was detected using the purified antibody.
Western blot of CRIP1 Antibody in mouse bladder tissue lysates (35 ug/lane). CRIP1 (arrow) was detected using the purified antibody.

Flow Cytometry

CRIP1 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CRIP1 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry - Paraffin

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot of CRIP1 Antibody in HL-60 cell line lysates (35 ug/lane). CRIP1 (arrow) was detected using the purified antibody.
Western blot of CRIP1 Antibody in HL-60 cell line lysates (35 ug/lane). CRIP1 (arrow) was detected using the purified antibody.

Western blot

Western blot of CRIP1 Antibody in mouse bladder tissue lysates (35 ug/lane). CRIP1 (arrow) was detected using the purified antibody.
Western blot of CRIP1 Antibody in mouse bladder tissue lysates (35 ug/lane). CRIP1 (arrow) was detected using the purified antibody.

Flow Cytometry

CRIP1 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CRIP1 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry - Paraffin

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot of CRIP1 Antibody in HL-60 cell line lysates (35 ug/lane). CRIP1 (arrow) was detected using the purified antibody.
Western blot of CRIP1 Antibody in HL-60 cell line lysates (35 ug/lane). CRIP1 (arrow) was detected using the purified antibody.

Western blot

Western blot of CRIP1 Antibody in mouse bladder tissue lysates (35 ug/lane). CRIP1 (arrow) was detected using the purified antibody.
Western blot of CRIP1 Antibody in mouse bladder tissue lysates (35 ug/lane). CRIP1 (arrow) was detected using the purified antibody.

Flow Cytometry

CRIP1 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CRIP1 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 9/20/2018

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