Human CREB1 / CREB

Mouse

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

Unconjugated

Protein G purified

Unmodified

Proprietary immunogen based on the amino acid sequence containing phospho-serine corresponding to residue 133 of human CREB.

pSer133

Recognizes phospho-CREB (Ser133), Mr 43kD. Species cross-reactivity: Rat and mouse. Broad species cross-reactivity expected, based on sequence homology.

• Western blot (1:1000)

Suitable for use in Western Blot. Western Blot: 1:1000 to 1:4000. Detects phospho-CREB (Ser133) in RIPA lysates from forskolin treated rat PC-12 and mouse 3T3/A31 cells. Lysates from untreated (Lanes 1 and 3) and forskolin treated (Lanes 2 and 4) PC-12 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-CREB (Ser133) (1:4000 dilution, Lanes 1 and 2) or anti-CREB (1:2000 dilution, Lanes 3 and 4). Proteins were visualized using a goat antimouse (Lanes 1and 2) or goat antirabbit (Lanes 3 and 4) secondary antibody conjugated to HRP and a chemiluminescence detection system.

0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P16220 NCBI: NM_134442 NP_004370.1