Human CR1 / CD35

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

E11

Unconjugated

Protein A purified

Preservative Free. Also available Unmodified.

Human acute monocytic leukaemia cells.

Is a single chain cell surface glycoprotein which exists in four allotypic forms (A, B, C, D) of 190kD, 220kD, 160kD and 250kD respectively. The molecule is expressed by granulocytes, monocytes, B cells and some T cells. Functionally CD35 acts as a receptor for the C3b component of complement. Clone E11 has been reported to be suitable for use in Western Blot.

• IHC
• IHC - Paraffin (10 µg/ml)
• IHC - Frozen
• Flow Cytometry (1:50 - 1:100)

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Immunohistochemistry: LS-B2556 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2556 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the diluted antibody to label 10^6 cells or 100 ul whole blood. Histology Positive Control Tissue: Tonsil.

PBS

Store at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P17927 NCBI: NM_000651 NP_000642.3