CD35 antibody LS-B2556 is an unconjugated mouse monoclonal antibody to CD35 (CR1) from human, baboon, cynomolgus monkey and other species. Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Human, Baboon, Cynomolgus monkey, Rhesus monkey
(tested or 100% immunogen sequence identity)
Protein A purified
Also available Unmodified
- IHC - Paraffin (10 µg/ml)
- IHC - Frozen
- Western blot
- Flow Cytometry (1:50 - 1:100)
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
CR1 / CD35 antibody was raised against human acute monocytic leukaemia cells.
Is a single chain cell surface glycoprotein which exists in four allotypic forms (A, B, C, D) of 190kD, 220kD, 160kD and 250kD respectively. The molecule is expressed by granulocytes, monocytes, B cells and some T cells. Functionally CD35 acts as a receptor for the C3b component of complement. Clone E11 has been reported to be suitable for use in Western Blot.
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Immunohistochemistry: LS-B2556 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2556 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the diluted antibody to label 10^6 cells or 100 ul whole blood. Histology Positive Control Tissue: Tonsil.
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.