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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CR1 / CD35 Antibody (clone E11, Azide‑free) IHC‑plus™ LS‑B2556
Note: This antibody replaces LS-C45218
CD35 antibody LS-B2556 is an unconjugated mouse monoclonal antibody to CD35 (CR1) from human, baboon, cynomolgus monkey and other species. Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2556-50
50 µg (1 mg/ml)
$425

Popular CR1 / CD35 Products

Antibody
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Antibody
Species: Human
Applications: ELISA
Antibody
Species: Human, Baboon, Monkey, Cynomolgus
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry
Anti-CD35 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Baboon, Cynomolgus monkey, Rhesus monkey
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry

Product Description

CD35 antibody LS-B2556 is an unconjugated mouse monoclonal antibody to CD35 (CR1) from human, baboon, cynomolgus monkey and other species. Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human CR1 / CD35
Synonyms
CR1, C3b/C4b receptor, C3-binding protein, CD35, C3BR, Knops blood group antigen, C4BR, CD35 antigen, Complement receptor type 1, KN
Host
Mouse
Reactivity
Human, Baboon, Cynomolgus monkey, Rhesus monkey (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [E11]
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Azide-free. Also available Unmodified.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen
  • Western blot
  • Flow Cytometry (1:50 - 1:100)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CR1 / CD35 antibody was raised against human acute monocytic leukaemia cells.
Specificity
Is a single chain cell surface glycoprotein which exists in four allotypic forms (A, B, C, D) of 190kD, 220kD, 160kD and 250kD respectively. The molecule is expressed by granulocytes, monocytes, B cells and some T cells. Functionally CD35 acts as a receptor for the C3b component of complement. Clone E11 has been reported to be suitable for use in Western Blot.
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Immunohistochemistry: LS-B2556 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2556 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the diluted antibody to label 10^6 cells or 100 ul whole blood. Histology Positive Control Tissue: Tonsil.
Presentation
PBS, pH 7.4
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About CR1 / CD35
CR1 / CD35 is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. P17927 NM_000651 NP_000642.3


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Images

Immunohistochemistry

Anti-CD35 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-CD35 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CD35 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-CD35 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CD35 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-CD35 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CD35 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-CD35 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CD35 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-CD35 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CD35 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-CD35 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CD35 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-CD35 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-CD35 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-CD35 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/15/2018

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