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Antibodies > CP / Ceruloplasmin > LS-C408064
 
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Catalog Number Size Price
LS-C408064-10 10 µg $318 
LS-C408064-100 100 µg $470 
CP / Ceruloplasmin Antibody - IHC analysis of Ceruloplasmin/CP using anti-Ceruloplasmin/CP antibody. Ceruloplasmin/CP was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Ceruloplasmin/CP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CP / Ceruloplasmin Antibody - Ceruloplasmin was detected in paraffin-embedded sections of mouse brain tissues using rabbit anti- Ceruloplasmin Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
CP / Ceruloplasmin Antibody - Ceruloplasmin was detected in paraffin-embedded sections of rat brain tissues using rabbit anti- Ceruloplasmin Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
CP / Ceruloplasmin Antibody - Western blot analysis of Ceruloplasmin/CP using anti-Ceruloplasmin/CP antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ceruloplasmin/CP antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Ceruloplasmin/CP at approximately 140KD. The expected band size for Ceruloplasmin/CP is at 122KD.
CP / Ceruloplasmin Antibody - Western blot analysis of Ceruloplasmin expression in rat skeletal muscle extract (lane 1), and NIH3T3 whole cell lysates (lane 2). Ceruloplasmin at 180 kD was detected using rabbit anti- Ceruloplasmin Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.
CP / Ceruloplasmin Antibody - IHC analysis of Ceruloplasmin/CP using anti-Ceruloplasmin/CP antibody. Ceruloplasmin/CP was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Ceruloplasmin/CP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CP / Ceruloplasmin Antibody - Ceruloplasmin was detected in paraffin-embedded sections of mouse brain tissues using rabbit anti- Ceruloplasmin Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
CP / Ceruloplasmin Antibody - Ceruloplasmin was detected in paraffin-embedded sections of rat brain tissues using rabbit anti- Ceruloplasmin Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
CP / Ceruloplasmin Antibody - Western blot analysis of Ceruloplasmin/CP using anti-Ceruloplasmin/CP antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ceruloplasmin/CP antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Ceruloplasmin/CP at approximately 140KD. The expected band size for Ceruloplasmin/CP is at 122KD.
CP / Ceruloplasmin Antibody - Western blot analysis of Ceruloplasmin expression in rat skeletal muscle extract (lane 1), and NIH3T3 whole cell lysates (lane 2). Ceruloplasmin at 180 kD was detected using rabbit anti- Ceruloplasmin Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.
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Polyclonal Rabbit anti‑Mouse CP / Ceruloplasmin Antibody (aa20‑258, IHC, WB) LS‑C408064

Polyclonal Rabbit anti‑Mouse CP / Ceruloplasmin Antibody (aa20‑258, IHC, WB) LS‑C408064

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Antibody:
CP / Ceruloplasmin Rabbit anti-Mouse Polyclonal (aa20-258) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C408064-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CP / Ceruloplasmin Rabbit anti-Mouse Polyclonal (aa20-258) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Ceruloplasmin antibody LS-C408064 is an unconjugated rabbit polyclonal antibody to Ceruloplasmin (CP) (aa20-258) from mouse. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Mouse CP / Ceruloplasmin
Synonyms
CP | Ceruloplasmin (ferroxidase) | Ceruloplasmin | CP-2 | Ferroxidase
Host
Rabbit
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived mouse Ceruloplasmin recombinant protein (Position: R20-M258). Mouse Ceruloplasmin shares 80.8% and 91.2% amino acid (aa) sequence identity with human and rat Ceruloplasmin, respectively.
Epitope
aa20-258
Specificity
Expressed in many tissues, including liver, eye and brain. .
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CP / Ceruloplasmin
P00450 NM_000096 NP_000087.1

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Applications: Western blot, ELISA
CP / Ceruloplasmin Antibody - Western Blot; Lane1: Rat Lung Tissue; Lane2: Rat Brain Tissue; Lane3: Rat Liver Tissue; Lane4: Rat Serum.
CP / Ceruloplasmin Antibody (aa872‑1059) LS‑C372630
Species: Rat
Applications: Western blot
CP / Ceruloplasmin Antibody - CP Antibody IHC of formalin-fixed and paraffin-embedded human hepatocarcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
CP / Ceruloplasmin Antibody (aa547‑577) LS‑C166975
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
CP / Ceruloplasmin Antibody - Anti-CP / Ceruloplasmin antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC‑plus™ CP / Ceruloplasmin Antibody LS‑B11410
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
CP / Ceruloplasmin ELISA Kit
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Reactivity: Human
Range: 1-1000 ng/ml
See all CP / Ceruloplasmin Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
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