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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CORNIFIN / SPRR1B Antibody (C‑Terminus) LS‑C146187 Best Seller
SPRR1B antibody LS-C146187 is an unconjugated rabbit polyclonal antibody to SPRR1B (CORNIFIN) from human and monkey. Validated for WB.
Catalog
Size
Price
LS-C146187-50
50 µg
$365

Popular CORNIFIN / SPRR1B Products

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with SPRR1B Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

SPRR1B antibody LS-C146187 is an unconjugated rabbit polyclonal antibody to SPRR1B (CORNIFIN) from human and monkey. Validated for WB.

Specifications

Target
Human CORNIFIN / SPRR1B
Synonyms
SPRR1B, 14.9 kd pancornulin, 14.9 kDa pancornulin, CORNIFIN, GADD33, SPR-IB, Small proline-rich protein IB, SPRR1, Cornifin B, Cornifin-B, Small proline-rich protein 1B
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide from C-Terminus of human SPRR1B (P22528, NP_003116). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%); Panda, Pig (92%); Mouse, Rat, Bovine (84%).
Blocking Peptide
LS-E23182 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C146187. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human SPRR1B
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About CORNIFIN / SPRR1B
Cross-linked envelope protein of keratinocytes. It is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane. Can function as both amine donor and acceptor in transglutaminase-mediated cross-linkage. P22528 NM_003125 NP_003116.2


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Images

Western blot

SPRR1B antibody Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SPRR1B antibody Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SPRR1B antibody Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SPRR1B antibody Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SPRR1B antibody Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SPRR1B antibody Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SPRR1B antibody Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SPRR1B antibody Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/15/2018

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