Beta-COP antibody LS-B2677 is an unconjugated rabbit polyclonal antibody to Beta-COP (COPB2) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Rat COPB2 / Beta-COP
Rat, Human, Monkey, Mouse, Bat, Bovine, Dog, Horse, Pig
(tested or 100% immunogen sequence identity)
Rabbit (at least 90% immunogen sequence identity)
- IHC - Paraffin (5 µg/ml)
- Western blot (2 µg/ml)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Synthetic peptide: KVLAAQETHEGVTEDGIE, corresponding to amino acids 494-511 of Rat beta COP I. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Platypus (100%); Orangutan, Rabbit, Lizard (94%); Chicken (89%); Turkey, Xenopus, Sea anemone (83%).
Detects rat coatomer-protein I beta (COP I beta).
Immunohistochemistry: LS-B2677 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2677 was determined to be 5 ug/ml. Western Blot: 2 ug/ml; Detects an ~115kD protein representing COP I beta from PC-12 cell extract.
PBS, 1 mg/ml BSA, 0.05% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.