Rat COPB2 / Beta-COP
Rat, Human, Monkey, Mouse, Bat, Bovine, Dog, Horse, Pig
(tested or 100% immunogen sequence identity)
Rabbit (at least 90% immunogen sequence identity)
- IHC - Paraffin (5 µg/ml)
- Western blot (2 µg/ml)
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Synthetic peptide: KVLAAQETHEGVTEDGIE, corresponding to amino acids 494-511 of Rat beta COP I. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Platypus (100%); Orangutan, Rabbit, Lizard (94%); Chicken (89%); Turkey, Xenopus, Sea anemone (83%).
Detects rat coatomer-protein I beta (COP I beta).
Immunohistochemistry: LS-B2677 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2677 was determined to be 5 ug/ml. Western Blot: 2 ug/ml; Detects an ~115kD protein representing COP I beta from PC-12 cell extract.
PBS, 1 mg/ml BSA, 0.05% sodium azide.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.
This antibody carries the LSBio 100% Guarantee.