Complement C8b antibody LS-C80646 is an unconjugated rabbit polyclonal antibody to Complement C8b from human, bat, bovine and other species. Validated for IHC and WB.
Human, Monkey, Bat, Bovine, Dog, Horse
(tested or 100% immunogen sequence identity)
Also available conjugated with FITC
Complement C8b antibody was raised against synthetic peptide located between aa321-370 of human C8B (P07358, NP_000057). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Dog, Bovine, Bat, Horse (100%); Mouse, Rat, Rabbit, Pig, Guinea pig (92%); Elephant, Turkey, Chicken, Lizard, Xenopus, Trout, Stickleback (85%).
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.5 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.