(tested or 100% immunogen sequence identity)
Complement C5 antibody was raised against purified human C5.
Recognizes secreted complement component 5 (C5), a 190kD serine protease present in blood serum. It is composed of an 115kD disulphide linked alpha-chain and a 75 kD beta-chain. In addition to its role in complement, C5 is thought to be associated with inflammation, stimulating tumor necrosis factor alpha, interferon gamma mediated suppression of IL10 and IL12 production. C5 is activated via either the classical or the alternative complement pathway, by proteolytic cleavage of C5a from the N-terminal of the alpha-chain, by C5 convertase. C5a is an anaphylatoxin which mediates local inflammation. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. It also stimulates chemotaxis of neutrophils, eosinophils, basophils and monocytes towards the site of inflammation. The remaining fragment, C5b, initiates the assembly of the membrane attack complex. In hemolysis inhibition studies it was found that Will block C5 hemolytic activity. Removal of sodium azide is recommended prior to use in functional assays. XXX XXX recommend the use of EUQOO3 for this purpose.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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