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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Complement C5 Antibody (aa1223‑1451) LS‑C186223
Complement C5 antibody LS-C186223 is an unconjugated rabbit polyclonal antibody to human Complement C5. Validated for IHC and WB.
Catalog
Size
Price
LS-C186223-100
100 µl (1 mg/ml)
$335
Description
Complement C5 antibody LS-C186223 is an unconjugated rabbit polyclonal antibody to human Complement C5. Validated for IHC and WB.
Target
Human Complement C5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Complement C5 antibody was raised against recombinant fragment corresponding to a region within amino acids 1223 and 1451 of C5 (SwissProt P01031). Percent identity by BLAST analysis: Human (100%); Pig (80%).
Epitope
aa1223-1451
Specificity
Human Complement C5
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
About Complement C5
P01031 NM_001735 NP_001726.2

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Images

Immunohistochemistry

IHC of paraffin-embedded hepatoma using C5 antibody antibody at 1:500 dilution.
IHC of paraffin-embedded hepatoma using C5 antibody antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: H1299. 5% SDS PAGE. Complement C5 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: H1299. 5% SDS PAGE. Complement C5 antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded hepatoma using C5 antibody antibody at 1:500 dilution.
IHC of paraffin-embedded hepatoma using C5 antibody antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: H1299. 5% SDS PAGE. Complement C5 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: H1299. 5% SDS PAGE. Complement C5 antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded hepatoma using C5 antibody antibody at 1:500 dilution.
IHC of paraffin-embedded hepatoma using C5 antibody antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: H1299. 5% SDS PAGE. Complement C5 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: H1299. 5% SDS PAGE. Complement C5 antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded hepatoma using C5 antibody antibody at 1:500 dilution.
IHC of paraffin-embedded hepatoma using C5 antibody antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: H1299. 5% SDS PAGE. Complement C5 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: H1299. 5% SDS PAGE. Complement C5 antibody diluted at 1:1000.

Requested From: United States
Date Requested: 12/17/2018
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