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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Complement C5 Antibody (aa1‑77) LS‑C344039
Note: This antibody replaces LS-C388108
Complement C5 antibody LS-C344039 is an unconjugated rabbit polyclonal antibody to rat Complement C5. Validated for IHC and WB.
Catalog
Size
Price
LS-C344039-100
100 µg (0.5 mg/ml)
$325

Popular Complement C5 Products

Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Mouse
Applications: Western blot
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA

Product Description

Complement C5 Antibody (aa1-77) for IHC, WB/Western LS-C344039

Specifications

Target
Rat Complement C5
Synonyms
C5, Anaphylatoxin C5a analog, CPAMD4, Complement C5, Complement component 5
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Complement C5 antibody was raised against e.coli-derived rat C5a recombinant protein (Position: D1-R77). Rat C5a shares 62% and 82% amino acid (aa) sequences identity with human and mouse C5a, respectively.
Epitope
aa1-77
Presentation
Contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2 HPO4, 0.05 mg sodium azide per 100 ug antibody.
Reconstitution
200 µl Distilled water
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid freeze-thaw cycles.
Restrictions
For research use only.
About Complement C5
Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. P01031 NM_001735 NP_001726.2


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Images

Immunohistochemistry

C5A antibody IHC-paraffin: Rat Spleen Tissue.
C5A antibody IHC-paraffin: Rat Spleen Tissue.

Western blot

C5A antibody Western blot. All lanes: Anti C5A at 0.5 ug/ml. WB: Recombinant Rat C5A Protein 0.5ng. Predicted band size: 37 kD. Observed band size: 37 kD.
C5A antibody Western blot. All lanes: Anti C5A at 0.5 ug/ml. WB: Recombinant Rat C5A Protein 0.5ng. Predicted band size: 37 kD. Observed band size: 37 kD.

Western blot

C5A antibody Western blot. All lanes: Anti C5A at 0.5 ug/ml. WB: Rat Liver Tissue Lysate at 50 ug. Predicted band size: 115 kD. Observed band size: 115 kD.
C5A antibody Western blot. All lanes: Anti C5A at 0.5 ug/ml. WB: Rat Liver Tissue Lysate at 50 ug. Predicted band size: 115 kD. Observed band size: 115 kD.

Immunohistochemistry

C5A antibody IHC-paraffin: Rat Spleen Tissue.
C5A antibody IHC-paraffin: Rat Spleen Tissue.

Western blot

C5A antibody Western blot. All lanes: Anti C5A at 0.5 ug/ml. WB: Recombinant Rat C5A Protein 0.5ng. Predicted band size: 37 kD. Observed band size: 37 kD.
C5A antibody Western blot. All lanes: Anti C5A at 0.5 ug/ml. WB: Recombinant Rat C5A Protein 0.5ng. Predicted band size: 37 kD. Observed band size: 37 kD.

Western blot

C5A antibody Western blot. All lanes: Anti C5A at 0.5 ug/ml. WB: Rat Liver Tissue Lysate at 50 ug. Predicted band size: 115 kD. Observed band size: 115 kD.
C5A antibody Western blot. All lanes: Anti C5A at 0.5 ug/ml. WB: Rat Liver Tissue Lysate at 50 ug. Predicted band size: 115 kD. Observed band size: 115 kD.

Immunohistochemistry

C5A antibody IHC-paraffin: Rat Spleen Tissue.
C5A antibody IHC-paraffin: Rat Spleen Tissue.

Western blot

C5A antibody Western blot. All lanes: Anti C5A at 0.5 ug/ml. WB: Recombinant Rat C5A Protein 0.5ng. Predicted band size: 37 kD. Observed band size: 37 kD.
C5A antibody Western blot. All lanes: Anti C5A at 0.5 ug/ml. WB: Recombinant Rat C5A Protein 0.5ng. Predicted band size: 37 kD. Observed band size: 37 kD.

Western blot

C5A antibody Western blot. All lanes: Anti C5A at 0.5 ug/ml. WB: Rat Liver Tissue Lysate at 50 ug. Predicted band size: 115 kD. Observed band size: 115 kD.
C5A antibody Western blot. All lanes: Anti C5A at 0.5 ug/ml. WB: Rat Liver Tissue Lysate at 50 ug. Predicted band size: 115 kD. Observed band size: 115 kD.

Immunohistochemistry

C5A antibody IHC-paraffin: Rat Spleen Tissue.
C5A antibody IHC-paraffin: Rat Spleen Tissue.

Western blot

C5A antibody Western blot. All lanes: Anti C5A at 0.5 ug/ml. WB: Recombinant Rat C5A Protein 0.5ng. Predicted band size: 37 kD. Observed band size: 37 kD.
C5A antibody Western blot. All lanes: Anti C5A at 0.5 ug/ml. WB: Recombinant Rat C5A Protein 0.5ng. Predicted band size: 37 kD. Observed band size: 37 kD.

Western blot

C5A antibody Western blot. All lanes: Anti C5A at 0.5 ug/ml. WB: Rat Liver Tissue Lysate at 50 ug. Predicted band size: 115 kD. Observed band size: 115 kD.
C5A antibody Western blot. All lanes: Anti C5A at 0.5 ug/ml. WB: Rat Liver Tissue Lysate at 50 ug. Predicted band size: 115 kD. Observed band size: 115 kD.

Requested From: United States
Date Requested: 10/17/2018

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