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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Complement C3 Antibody (clone 5F9) IHC‑plus™ LS‑B4290
Note: This antibody replaces LS-C104871
Complement C3 antibody LS-B4290 is an unconjugated mouse monoclonal antibody to human Complement C3. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B4290-50
50 µg (0.5 mg/ml)
$485
Description
Complement C3 antibody LS-B4290 is an unconjugated mouse monoclonal antibody to human Complement C3. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human Complement C3
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [5F9]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Complement C3 antibody was raised against c3 (NP_000055, 1534 a.a. ~ 1645 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Usage
Western Blot (Cell lysate) - positive control A-431. Sandwich ELISA (Recombinant protein).
Presentation
PBS, pH 7.4
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
About Complement C3
P01024 NM_000064 NP_000055.2

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Images

Immunohistochemistry

Anti-Complement C3 antibody IHC of human vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Complement C3 antibody IHC of human vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

C3 monoclonal antibody, clone 5F9 Western blot of C3 expression in A-431.
C3 monoclonal antibody, clone 5F9 Western blot of C3 expression in A-431.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged C3 is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged C3 is approximately 1 ng/ml as a capture antibody.

Immunohistochemistry

Anti-Complement C3 antibody IHC of human vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Complement C3 antibody IHC of human vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

C3 monoclonal antibody, clone 5F9 Western blot of C3 expression in A-431.
C3 monoclonal antibody, clone 5F9 Western blot of C3 expression in A-431.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged C3 is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged C3 is approximately 1 ng/ml as a capture antibody.

Immunohistochemistry

Anti-Complement C3 antibody IHC of human vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Complement C3 antibody IHC of human vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

C3 monoclonal antibody, clone 5F9 Western blot of C3 expression in A-431.
C3 monoclonal antibody, clone 5F9 Western blot of C3 expression in A-431.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged C3 is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged C3 is approximately 1 ng/ml as a capture antibody.

Immunohistochemistry

Anti-Complement C3 antibody IHC of human vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Complement C3 antibody IHC of human vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

C3 monoclonal antibody, clone 5F9 Western blot of C3 expression in A-431.
C3 monoclonal antibody, clone 5F9 Western blot of C3 expression in A-431.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged C3 is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged C3 is approximately 1 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 12/12/2018
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