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Catalog Number Size Price
LS-C745506-50 50 µg (1 mg/ml) $582 
Collagen VI Antibody - Immunohistochemistry of rabbit anti-collagen VI antibody. Tissue: human placenta (left), testis (right). Fixation: formalin fixed paraffin embedded. Antigen retrieval: steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Primary antibody: collagen VI antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: collagen VI is extracellular. Staining: collagen VI as precipitated red signal red staining of stromal and extracellular spaces in the placenta and extracellular spaces between seminiferous tubules in the testis, with hematoxylin purple nuclear counterstain.

Polyclonal Rabbit anti‑Mammal Collagen VI Antibody (FITC, IHC, WB) LS‑C745506

Polyclonal Rabbit anti‑Mammal Collagen VI Antibody (FITC, IHC, WB) LS‑C745506

Antibody:
Collagen VI Rabbit anti-Mammal Polyclonal (FITC) Antibody
Application:
IHC, WB, IP, ELISA
Reactivity:
Mammal, Human, Bovine
Format:
FITC, Unmodified
Price
Catalog Number
$582
LS-C745506-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Collagen VI Rabbit anti-Mammal Polyclonal (FITC) Antibody
Application:
IHC, WB, IP, ELISA
Reactivity:
Mammal, Human, Bovine
Format:
FITC, Unmodified

Specifications

Description
Collagen VI antibody LS-C745506 is an FITC-conjugated rabbit polyclonal antibody to Collagen VI from mammal. It is reactive with human, bovine and mammal. Validated for ELISA, IHC, IP and WB.
Target
Mammal Collagen VI
Host
Rabbit
Reactivity
Mammal, Human, Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
FITC
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Collagen Type VI from human and bovine placenta.
Specificity
Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Applications
  • IHC (1:200 - 1:1000)
  • Western blot (1:1000 - 1:5000)
  • Immunoprecipitation (1:100)
  • ELISA (1:10000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.01 M Sodium Phosphate, pH 7.2, 0.25 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 50µL of deionized water.
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 4/25/2024