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Catalog Number Size Price
LS-C744078-0.5 0.5 ml (1 mg/ml) $879 
Collagen VI Antibody - Collagen Type VI on human skin paraffin section DAB, hematoxylin

Polyclonal Rabbit anti‑Human Collagen VI Antibody (IHC, IF, WB) LS‑C744078

Polyclonal Rabbit anti‑Human Collagen VI Antibody (IHC, IF, WB) LS‑C744078

Antibody:
Collagen VI Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, IF, WB, ELISA, RIA
Reactivity:
Human, Mouse, Rat, Pig, Chicken, Cow
Format:
Unconjugated, Unmodified
Price
Catalog Number
$879
LS-C744078-0.5
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Collagen VI Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, IF, WB, ELISA, RIA
Reactivity:
Human, Mouse, Rat, Pig, Chicken, Cow
Format:
Unconjugated, Unmodified

Specifications

Description
Collagen VI antibody LS-C744078 is an unconjugated rabbit polyclonal antibody to Collagen VI from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC, RIA and WB.
Target
Human Collagen VI
Host
Rabbit
Reactivity
Human, Mouse, Rat, Pig, Chicken, Cow (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
Purified collagen type VI from human placenta
Applications
  • IHC - Paraffin (1:1000)
  • Immunofluorescence (1:80)
  • Western blot
  • ELISA (1:200)
  • Radioimmunoassay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Collagen type VI is found as 5-10 nm diameter microfibrils (periodicity 100 nm) e.g. in placenta, skin, cartilage and cornea. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human collagen type VI 100%, human collagen type I, II, III, IV and V <0.1%; human elastin and human laminin <0.1%; human fibronectin < 1% (RIA at 1:10.000 dilution).
Presentation
PBS
Reconstitution
0.5 ml Reconstitute in sterile distilled water.
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 1/25/2022